IgA Secretory Component / ECM1 Mouse Monoclonal Antibody | MyBioSource MBS438012 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

IgA Secretory Component / ECM1 Mouse Monoclonal Antibody

catalog :

MBS438012

quantity :

0.02 mg (With BSA &

price :

150 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

SPM217

reactivity :

human, rat

application :

immunohistochemistry, immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS438012

products type :

Antibody

products full name :

IgA Secretory Component / ECM1 Mouse Monoclonal Antibody

products short name :

IgA Secretory Component / ECM1

products name syn :

ECM1, Extracellular Matrix Protein 1, Secretory Component p85, URBWD

other names :

extracellular matrix protein 1 isoform 3; Extracellular matrix protein 1; extracellular matrix protein 1; extracellular matrix protein 1; Secretory component p85

products gene name :

ECM1

other gene names :

ECM1; ECM1; URBWD

uniprot entry name :

ECM1_HUMAN

clonality :

Monoclonal

isotype :

IgG1, kappa

clone :

SPM217

host :

Mouse

reactivity :

Human, Rat

sequence length :

567

specificity :

This MAb reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.

form :

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

storage stability :

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

tested application :

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

app notes :

Flow Cytometry (0.5-1ug/million cells in 0.1ml). Immunofluorescence (1-2ug/ml). Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

other info1 :

Cellular Localization: Cell Surface and Cytoplasmic. Immunogen: Secretory Component protein isolated from human colostrum

other info2 :

Hu-Chromosome Location: 1q21.2. Positive Control: Stomach, Lung, or breast tumor

products references :

Bartek J et. al. Histochemistry 91:235-244 (1989)

ncbi gi num :

322302700

ncbi acc num :

NP_001189787.1

ncbi gb acc num :

NM_001202858.1

uniprot acc num :

Q16610

ncbi mol weight :

~80kDa

ncbi pathways :

Hemostasis Pathway (1269340); Platelet Activation, Signaling And Aggregation Pathway (1269350); Platelet Degranulation Pathway (1269367); Response To Elevated Platelet Cytosolic Ca2+ Pathway (1269365)

ncbi summary :

This gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Feb 2011]

uniprot summary :

ECM1: Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity. Defects in ECM1 are the cause of lipoid proteinosis (LiP); also known as lipoid proteinosis of Urbach and Wiethe or hyalinosis cutis et mucosae. LiP is a rare autosomal recessive disorder characterized by generalized thickening of skin, mucosae and certain viscera. Classical features include beaded eyelid papules and laryngeal infiltration leading to hoarseness. Histologically, there is widespread deposition of hyaline material and disruption/reduplication of basement membrane. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: extracellular matrix; extracellular region. Molecular Function: enzyme binding; laminin binding; protease binding; protein binding; protein C-terminus binding; signal transducer activity. Biological Process: negative regulation of bone mineralization; negative regulation of peptidase activity; platelet degranulation; positive regulation of angiogenesis; positive regulation of endothelial cell proliferation; positive regulation of I-kappaB kinase/NF-kappaB cascade. Disease: Lipoid Proteinosis Of Urbach And Wiethe

size1 :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price1 :

150 USD

size2 :

7 ml (Prediluted)

price2 :

270

size3 :

0.1 mg (With BSA & Azide at 0.2mg/ml)

price3 :

270

size4 :

0.1 mg (Without BSA & Azide at 1mg/ml)

price4 :

285

size5 :

0.2 mg (With BSA & Azide at 0.2mg/ml)

price5 :

360

more info or order :

MyBioSource product webpage