other

KlenThermase DNA Polymerase

MBS433679

1000 Units

390 USD

Taq DNA Polymerase

KlenThermase DNA Polymerase

[KlenThermase DNA Polymerase]

[KlenThermaseTM DNA Polymerase]

KlenThermase TMDNA polymerase(25 U/?l), 1000 Units

25 units/ul.

Store KlenThermase™ DNA polymerase below 0°C, preferably at -20°C, in a constant temperature freezer.Avoid repeated freeze-thaw. Shelf life:18 months from date of receipt under proper storage conditions (-20°C)

Designed for manual DNA sequencing with 35S label and for automated fluorescent DNA sequencing.

Functional Analysis: Tested functionally in a unit activity test.

Reaction Buffer: 500 mM KCl, 100 mM Tris-HCl (pH 9 at 25°C), 1% Triton X100. Extra solution: 50 mM MgCl2, add MgCl2 to a final concentration of 3.5 mM. Buffers Not provided. Storage Buffer: 10 mM K-phosphate buffer pH 7.0, 100 mM NaCI, 0.5 mM EDTA, 1 mM DTT, 0.01% Tween 20; 50% glycerol (v/v). Unit Definition: One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs into acid-insoluble form in 30 minutes at 72°C under the assay conditions (25 mM TAPS (tris-(hydroxymethyl)-methyl-aminopropane-sulfonic acid, sodium salt) pH 9.3 (at 25°C), 50 mM KCI, 2 mM MgCl2, 1 mM.-mercaptoethanol and activated calf thymus DNA as substrate. Features: Fidelity: The relative mutation rate during polymerisation is twofold lower for KlenThermase™ as compared to the full-length Taq DNA polymerase. Cycle sequencing: The absence of the 5'-3' exonuclease activity makes KlenThermase™ especially suitable for cycle sequencing. It gives higher sequence intensity and very low backgrounds. The mutational optimization improves the uniformity of band intensities. Combination of KlenThermase™ with Tth inorganic pyrophospatase generates uniform bands that improve sequencing accuracy and give long read lengths.

Taq DNA Polymerases

Description: KlenThermase DNA polymerase is an optimized version of DNA polymerase designed for cycle sequencing with dideoxynucleotides. KlenThermase DNA polymerase is an optimised version of KlenTherm DNA polymerase designed for cycle sequencing with dideoxynucleotides. This enzyme is recommended both for manual DNA sequencing with 35S label and for automated fluorescent DNA sequencing. Mutations have been introduced into the KlenTherm DNA polymerase that confer on this enzyme enhanced properties for cycle sequencing of double-stranded PCR products. KlenThermase is similar to, yet distinct from, USB ThermoSequenase. We recommend to use KlenThermase with our thermostable Tth inorganic pyrophosphatase (1 unit of Tth inorganic pyrophosphatase added to 10 units of KlenThermase) for further improvement of uniformity of band intensities.

1000 Units

390 USD