Goat Anti-SNAP25, Biotinylated Antibody | MyBioSource MBS423684 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat Anti-SNAP25, Biotinylated Antibody

catalog :

MBS423684

quantity :

0.1 mg

price :

305 USD

clonality :

polyclonal

host :

goat

conjugate :

biotin

reactivity :

human, mouse, rat, dog

application :

western blot, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Biotinylated (0.1ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

product information

catalog number :

MBS423684

products type :

Antibody

products full name :

Goat Anti-SNAP25, Biotinylated Antibody

products short name :

[SNAP25]

products name syn :

[Goat Anti-SNAP25 Antibody; SNAP25 antibody; synaptosome associated protein 25kDa antibody; CMS18 antibody; RIC-4 antibody; RIC4 antibody; SEC9 antibody; SNAP antibody; SNAP-25 antibody; SUP antibody; bA416N4.2 antibody; dJ1068F16.2 antibody; resistance to inhibitors of cholinesterase 4 homolog antibody; synaptosomal-associated protein, 25kDa antibody]

other names :

[synaptosomal-associated protein 25 isoform SNAP25A; Synaptosomal-associated protein 25; synaptosomal-associated protein 25; synaptosome associated protein 25; Super protein; SUP; Synaptosomal-associated 25 kDa protein]

products gene name :

[SNAP25]

other gene names :

[SNAP25; SNAP25; SUP; RIC4; SEC9; SNAP; CMS18; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2; SNAP; SNAP-25; SUP]

uniprot entry name :

SNP25_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human, Mouse, Rat. Expected from sequence similarity: Human, Mouse, Rat, Dog

sequence length :

206

sequence :

DEANQRATKMLGSG.

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Western Blot: Approx 26kDa band observed in Rat Brain lysates (calculated MW of 23.3kDa according to NP_003072.2 and NP_570824.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3ug/ml. Peptide ELISA: Antibody detection limit dilution 1:64000.

image1 heading :

Western Blot (WB)

other info1 :

Immunogen: Peptide with sequence C-DEANQRATKMLGSG., from the C Terminus of the protein sequence according to NP_003072.2; NP_570824.1. Epitope: C Terminus

other info2 :

Note: This antibody is expected to recognise both reported isoforms NP_003072.2 (SNAP25A) and NP_570824.1 (SNAP25B). Reported variants represent identical protein: NP_001309831.1, NP_003072.2 Reported variants represent identical protein: NP_001309834.1, NP_001309832.1, NP_001309833.1, NP_001309839.1, NP_001309836.1, NP_570824.1, NP_001309838.1, NP_001309835.1, NP_001309837.1

ncbi gi num :

18765733

ncbi acc num :

NP_003072.2

ncbi gb acc num :

NM_003081.4

ncbi mol weight :

23,336 Da

ncbi pathways :

Acetylcholine Neurotransmitter Release Cycle Pathway (1268773); Disease Pathway (1268854); Dopamine Neurotransmitter Release Cycle Pathway (1268772); Effects Of Botulinum Toxin Pathway (138028); GABA Synthesis, Release, Reuptake And Degradation Pathway (1268774); Glutamate Neurotransmitter Release Cycle Pathway (1268771); Infectious Disease Pathway (1269056); Insulin Signaling Pathway (198845); Insulin Secretion Pathway (777534); Integration Of Energy Metabolism Pathway (1270101)

ncbi summary :

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

SNAP-25: a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Part of the SNARE core complex containing SNAP25, VAMP2 and syntaxin 1A. Associates with proteins involved in vesicle docking and membrane fusion. Two alternatively spliced isoforms have been described. Protein type: Vesicle. Chromosomal Location of Human Ortholog: 20p12-p11.2. Cellular Component: cytoplasm; cytosol; growth cone; membrane; neuron projection; plasma membrane; presynaptic membrane; SNARE complex; vesicle; voltage-gated potassium channel complex. Molecular Function: calcium-dependent protein binding; protein binding; SNAP receptor activity; syntaxin-1 binding. Biological Process: glutamate secretion; neurotransmitter secretion; regulation of insulin secretion; synaptic vesicle exocytosis; synaptic vesicle fusion to presynaptic membrane; synaptic vesicle priming. Disease: Myasthenic Syndrome, Congenital, 18

size1 :

0.1 mg

price1 :

305 USD

more info or order :

MyBioSource product webpage