Goat anti-Prestin (aa399-411) Antibody | MyBioSource MBS423494 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-Prestin (aa399-411) Antibody

catalog :

MBS423494

quantity :

0.1 mg

price :

225 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, cow

application :

ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS423494

products type :

Antibody

products full name :

Goat anti-Prestin (aa399-411) Antibody

products short name :

[Prestin]

products name syn :

[Slc26a5, solute carrier family 26, member 5, Pres, prestin, outer hair cell motor protein, prestin (motor protein)]

other names :

[prestin isoform 1; Prestin; prestin; solute carrier family 26, member 5; Solute carrier family 26 member 5]

products gene name :

[Slc26a5]

other gene names :

[Slc26a5; Slc26a5; Pres; prestin; Pres]

uniprot entry name :

S26A5_MOUSE

clonality :

Polyclonal

host :

Goat

reactivity :

Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

sequence length :

744

sequence :

RSLVQEGTGGKTQ

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Not yet tested. At this stage we are dependent on researchers in the field for further characterization of this product. Therefore we cannot recommend an optimal concentration and the antibody is an aspiring product.

other info1 :

Immunogen: Peptide with sequence C-RSLVQEGTGGKTQ, from the internal region of the protein sequence according to NP_109652.3. Epitope: Internal region

other info2 :

Note: The immunizing peptide represents part of an extracellular loop. Non-Human GeneID(s): 80979 (mouse), 83891 (rat)

ncbi gi num :

28933459

ncbi acc num :

NP_109652.3

ncbi gb acc num :

NM_030727.5

uniprot summary :

SLC26A5: Motor protein that converts auditory stimuli to length changes in outer hair cells and mediates sound amplification in the mammalian hearing organ. Prestin is a bidirectional voltage- to-force converter, it can operate at microsecond rates. It uses cytoplasmic anions as extrinsic voltage sensors, probably chloride and bicarbonate. After binding to a site with millimolar affinity, these anions are translocated across the membrane in response to changes in the transmembrane voltage. They move towards the extracellular surface following hyperpolarization, and towards the cytoplasmic side in response to depolarization. As a consequence, this translocation triggers conformational changes in the protein that ultimately alter its surface area in the plane of the plasma membrane. The area decreases when the anion is near the cytoplasmic face of the membrane (short state), and increases when the ion has crossed the membrane to the outer surface (long state). So, it acts as an incomplete transporter. It swings anions across the membrane, but does not allow these anions to dissociate and escape to the extracellular space. Salicylate, an inhibitor of outer hair cell motility, acts as competitive antagonist at the prestin anion-binding site. Defects in SLC26A5 are the cause of deafness autosomal recessive type 61 (DFNB61). A form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Belongs to the SLC26A/SulP transporter (TC 2.A.53) family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Transporter; Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral. Cellular Component: basolateral plasma membrane; cytoplasm; integral to membrane; integral to plasma membrane; lateral plasma membrane; membrane; plasma membrane. Molecular Function: anion:anion antiporter activity; bicarbonate transmembrane transporter activity; chloride channel activity; oxalate transmembrane transporter activity; protein binding; protein homodimerization activity; secondary active sulfate transmembrane transporter activity; spectrin binding; sulfate transmembrane transporter activity; transcription factor binding. Biological Process: bicarbonate transport; fructose transport; positive regulation of cell size; protein tetramerization; regulation of cell shape; regulation of intracellular pH; regulation of membrane potential; sensory perception of sound; sulfate transport; transmembrane transport

size1 :

0.1 mg

price1 :

225 USD

more info or order :

MyBioSource product webpage