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company name :
MyBioSource
product type :
antibody
product name :
Goat anti-EEA1 (aa821-835) Antibody
catalog :
MBS423213
quantity :
0.1 mg
price :
305 USD
clonality :
polyclonal
host :
goat
conjugate :
nonconjugated
reactivity :
human, mouse
application :
western blot, enzyme immunoassay
more info or order :
image
image 1 :
(0.3ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
image 2 :
Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml)
product information
catalog number :
MBS423213
products type :
Antibody
products full name :
Goat anti-EEA1 (aa821-835) Antibody
products short name :
[EEA1]
products name syn :
[EEA1; early endosome antigen 1; MST105; MSTP105; ZFYVE2; early endosome antigen 1, 162kD; early endosome-associated protein; endosome-associated protein p162; zinc finger FYVE domain-containing protein 2; early endosome antigen 1 antibody; early endosome antigen 1; 162kD antibody; early endosome-associated protein antibody; EEA1 antibody; endosome-associated protein p162 antibody; MST105 antibody; MSTP105 antibody; ZFYVE2 antibody; zinc finger FYVE domain-containing protein 2 antibody; EEA1 (aa821-835)]
other names :
[early endosome antigen 1; Early endosome antigen 1; early endosome antigen 1; early endosome antigen 1; Endosome-associated protein p162; Zinc finger FYVE domain-containing protein 2]
products gene name :
[EEA1]
other gene names :
[EEA1; EEA1; MST105; ZFYVE2; MSTP105; ZFYVE2]
uniprot entry name :
EEA1_HUMAN
clonality :
Polyclonal
host :
Goat
reactivity :
Tested: Human, Mouse. Expected from sequence similarity: Human
sequence length :
1411
sequence :
QETKIQHEELNNRIQ
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB)
app notes :
Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 200kDa band observed in lysates of cell lines A439 and HEK293 (calculated MW of 162kDa according to NP_003557.2). Recommended concentration: 0.3-1ug/ml. Immunofluorescence : Strong expression of the protein seen in the vesicles/cytoplasm of NIH3T3 cells. Recommended concentration: 5ug/ml.
image1 heading :
Western Blot (WB)
image2 heading :
Immunofluorescence
other info1 :
Immunogen: Peptide with sequence C-QETKIQHEELNNRIQ, from the internal region of the protein sequence according to NP_003557.2. Epitope: Internal region
ncbi gi num :
55770888
ncbi acc num :
NP_003557.2
ncbi gb acc num :
NM_003566.3
ncbi mol weight :
162,466 Da
ncbi pathways :
Endocytosis Pathway (102279); Endocytosis Pathway (102181); Immune System Pathway (1269170); Innate Immune System Pathway (1269203); Phagosome Pathway (153910); Phagosome Pathway (153859); Toll Like Receptor 9 (TLR9) Cascade Pathway (1269233); Toll-Like Receptors Cascades Pathway (1269204); Tuberculosis Pathway (213780); Tuberculosis Pathway (213743)
uniprot summary :
EEA1: Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking. Homodimer. Binds STX6. Binds RAB5A, RAB5B, RAB5C and RAB22A that have been activated by GTP-binding. Interacts with ERBB2. Protein type: C2H2-type zinc finger protein; Vesicle. Chromosomal Location of Human Ortholog: 12q22. Cellular Component: cytoplasm; cytosol; early endosome; extrinsic to plasma membrane; membrane. Molecular Function: GTP-dependent protein binding; phosphatidylinositol binding; protein binding; protein homodimerization activity; zinc ion binding. Biological Process: endocytosis; synaptic vesicle to endosome fusion; vesicle fusion
size1 :
0.1 mg
price1 :
305 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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