Goat anti-MED12 (aa1981-1994) Antibody | MyBioSource MBS423100 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-MED12 (aa1981-1994) Antibody

catalog :

MBS423100

quantity :

0.1 mg

price :

225 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, cow

application :

ELISA, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS423100

products type :

Antibody

products full name :

Goat anti-MED12 (aa1981-1994) Antibody

products short name :

[MED12]

products name syn :

[MED12; mediator complex subunit 12; ARC240; CAGH45; FGS1; HOPA; KIAA0192; OKS; OPA1; TNRC11; TRAP230; CAG repeat protein 45; OPA-containing protein; OTTHUMP00000023490; OTTHUMP00000023493; activator-recruited cofactor 240 kDa component; human opposite pai; MED12 antibody; mediator complex subunit 12 antibody; ARC240 antibody; CAGH45 antibody; FGS1 antibody; HOPA antibody; KIAA0192 antibody; OKS antibody; OPA1 antibody; TRAP230 antibody; CAG repeat protein 45 antibody; OPA-containing protein antibody; OTTHUMP00000023490 antibody; OTTHUMP00000023493 antibody; activator-recruited cofactor 240 kDa component antibody; human opposite pai antibody; MED12 (aa1981-1994)]

other names :

[mediator of RNA polymerase II transcription subunit 12; Mediator of RNA polymerase II transcription subunit 12; mediator of RNA polymerase II transcription subunit 12; mediator complex subunit 12; Activator-recruited cofactor 240 kDa component; ARC240; CAG repeat protein 45; Mediator complex subunit 12; OPA-containing protein; Thyroid hormone receptor-associated protein complex 230 kDa component; Trap230; Trinucleotide repeat-containing gene 11 protein]

products gene name :

[MED12]

other gene names :

[MED12; MED12; OKS; FGS1; HOPA; OPA1; OHDOX; ARC240; CAGH45; MED12S; TNRC11; TRAP230; ARC240; CAGH45; HOPA; KIAA0192; TNRC11; TRAP230; ARC240; Trap230]

uniprot entry name :

MED12_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

sequence length :

2177

sequence :

NPTLVDPTRHLQQR

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

storage stability :

Aliquot and store at -20°C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Preliminary experiments gave an approx 70kDa band in Human Brain (Olfactory Bulb) and Rat Brain lysates after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 243kDa according to NP_005111.2. The 70kDa band was successfully blocked by incubation with the immunizing peptide.

other info1 :

Immunogen: Peptide with sequence C-NPTLVDPTRHLQQR, from the internal region (near C Terminus) of the protein sequence according to NP_005111.2. Epitope: Internal region (near C Terminus)

ncbi gi num :

110347429

ncbi acc num :

NP_005111.2

ncbi gb acc num :

NM_005120.2

ncbi mol weight :

242,953 Da

ncbi pathways :

Developmental Biology Pathway (1270302); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (1270010); Gene Expression Pathway (1269649); Generic Transcription Pathway (1269650); Metabolism Pathway (1269956); Metabolism Of Lipids And Lipoproteins Pathway (1270001); PPARA Activates Gene Expression Pathway (1270030); Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway (169352); Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway (1270029); Thyroid Hormone Signaling Pathway (946598)

ncbi summary :

The initiation of transcription is controlled in part by a large protein assembly known as the preinitiation complex. A component of this preinitiation complex is a 1.2 MDa protein aggregate called Mediator. This Mediator component binds with a CDK8 subcomplex which contains the protein encoded by this gene, mediator complex subunit 12 (MED12), along with MED13, CDK8 kinase, and cyclin C. The CDK8 subcomplex modulates Mediator-polymerase II interactions and thereby regulates transcription initiation and reinitation rates. The MED12 protein is essential for activating CDK8 kinase. Defects in this gene cause X-linked Opitz-Kaveggia syndrome, also known as FG syndrome, and Lujan-Fryns syndrome. [provided by RefSeq, Aug 2009]

uniprot summary :

MED12: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Also interacts with CTNNB1 and GLI3. Ubiquitous. Belongs to the Mediator complex subunit 12 family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator. Chromosomal Location of Human Ortholog: Xq13. Cellular Component: centrosome; membrane; nucleolus; nucleoplasm; nucleus; Srb-mediator complex; ubiquitin ligase complex. Molecular Function: beta-catenin binding; chromatin binding; ligand-dependent nuclear receptor transcription coactivator activity; protein binding; protein C-terminus binding; protein domain specific binding; receptor activity; thyroid hormone receptor binding; transcription coactivator activity; transcription cofactor activity; vitamin D receptor binding. Biological Process: androgen receptor signaling pathway; embryonic neurocranium morphogenesis; endoderm development; heart development; negative regulation of Wnt receptor signaling pathway; neural tube closure; oligodendrocyte development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein ubiquitination; Schwann cell development; spinal cord development; stem cell maintenance; steroid hormone receptor signaling pathway; transcription initiation from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; Wnt receptor signaling pathway, planar cell polarity pathway. Disease: Lujan-fryns Syndrome; Ohdo Syndrome, X-linked; Opitz-kaveggia Syndrome

size1 :

0.1 mg

price1 :

225 USD

more info or order :

MyBioSource product webpage