Goat anti-calbindin D28 Antibody | MyBioSource MBS423038 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-calbindin D28 Antibody

catalog :

MBS423038

quantity :

0.1 mg

price :

305 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, cow

application :

western blot, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

MBS423038 (0.03ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

image 2 :

MBS423038 (0.1ug/ml) staining of Mouse (A) and Rat (B) Brain lysates (35?g protein in RIPA buffer). Detected by chemiluminescence.

image 3 :

MBS423038 (0.1ug/ml) staining of PFA-perfused cryosection of Human Hypothalamus. Antigen retrieval with citrate buffer pH 6 at 80C for 30min, HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.

product information

catalog number :

MBS423038

products type :

Antibody

products full name :

Goat anti-calbindin D28 Antibody

products short name :

[calbindin D28]

products name syn :

[CALB, CALB1, calbindin, calbindin 1, (28kD), calbindin 1, 28kDa, calbindin D28, D-28K, OTTHUMP00000166027, OTTHUMP00000225441, RTVL-H protein, vitamin D-dependent calcium-binding protein, avian-type]

other names :

[calbindin; Calbindin; calbindin; calbindin 1; Calbindin D28; D-28K; Vitamin D-dependent calcium-binding protein, avian-type]

products gene name :

[CALB1]

other gene names :

[CALB1; CALB1; CALB; D-28K; CAB27]

uniprot entry name :

CALB1_HUMAN

clonality :

polyclonal

host :

goat

reactivity :

Tested: Human, Mouse, Rat. Expected from sequence similarity: Human, Rat, Dog, Pig, Cow

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 26kDa band observed in Human Brain (Cerebellum) lysates and in Mouse and Rat total Brain lysates (calculated MW of 30.0kDa according to NP_004920.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Parmentier et al, Eur J Biochem. 1987 Dec30;170(1-2):207-15. PMID: 3691519. Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig Brain and Kidney for this particular batch. IHC: Frozen sections of Human Hypothalamus shows staining of Calbinding D2 neurons. Recommended concentration: 0.1-0.3ug/ml. Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

MBS423038 (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

other info1 :

Immunogen: Peptide with sequence C-KTFVDQYGQRDDGK, from the internal region of the protein sequence according to NP_004920.1.

ncbi gi num :

4826655

ncbi acc num :

NP_004920.1

ncbi gb acc num :

NM_004929.3

ncbi pathways :

Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213307); Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213276)

ncbi summary :

The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease. [provided by RefSeq, Jan 2015]

uniprot summary :

CALB1: Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. Interacts with RANBP9. Belongs to the calbindin family. Protein type: Calcium-binding. Chromosomal Location of Human Ortholog: 8q21.3. Cellular Component: axon; cell soma; cytosol; dendrite; nucleus; synapse; terminal button. Molecular Function: calcium ion binding; protein binding; vitamin D binding; zinc ion binding. Biological Process: cytosolic calcium ion homeostasis; locomotory behavior; long-term memory; regulation of synaptic plasticity; short-term memory

size1 :

0.1 mg

price1 :

305 USD

more info or order :

MyBioSource product webpage