antibody

Goat anti-Lamin B1 (isoform 1) Antibody

MBS422963

0.1 mg

305 USD

polyclonal

goat

nonconjugated

human, cow

western blot, enzyme immunoassay

Antibody

Goat anti-Lamin B1 (isoform 1) Antibody

[Lamin B1]

[ADLD, lamin B1, lamin-B1, LMN, LMN2, LMNB, LMNB1, MGC111419, OTTHUMP00000159218]

[lamin-B1 isoform 1; Lamin-B1; lamin-B1; lamin B1]

[LMNB1]

[LMNB1; LMNB1; LMN; ADLD; LMN2; LMNB; LMN2; LMNB]

LMNB1_HUMAN

Polyclonal

Goat

Tested: Human; Expected from sequence similarity: Human, Pig, Cow

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB)

Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 65kDa band observed in lysates of cell line Jurkat (calculated MW of 66.4kDa according to NP_005564.1). Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB)

Immunogen: Peptide with sequence C-KESDLNGAQIKLRE, from the internal region of the protein sequence according to NP_005564.1. Note: This antibody is expected to recognize isoform 1 (NP_005564.1) only. Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-6217

5031877

NP_005564.1

NM_005573.3

Apoptosis Pathway (83060); Apoptosis Pathway (470); Apoptosis Pathway (1270262); Apoptotic Cleavage Of Cellular Proteins Pathway (1270288); Apoptotic Execution Phase Pathway (1270287); Breakdown Of The Nuclear Lamina Pathway (1270291); Caspase Cascade In Apoptosis Pathway (137974); Cell Cycle Pathway (1269741); Cell Cycle, Mitotic Pathway (1269763); Cellular Senescence Pathway (1270426)

This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Lamin B1: nuclear lamins are intermediate filament proteins that constitute the lattice-like matrix at the inner face of the nuclear membrane that underlies the nuclear envelop. The lamins, highly conserved throughout evolution, are encoded by three genes in the human: LMNA, LMNB1, and LMNB2. The A-type lamins (lamin A/C) are developmentally regulated and are generally expressed in differentiated cells. The anchoring of chromatin to the nuclear lamina is involved in the control of gene expression and in DNA replication and repair. During mitosis, the nuclear lamina is reversibly disassembled as the lamin proteins are phosphorylated. B-type lamins are phosphorylated within minutes of engaging the IgM surface receptor of resting splenic B cells. Nuclear lamins are cleaved by caspases during apoptosis. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 5q23.2. Cellular Component: lamin filament; membrane; nuclear envelope; nuclear inner membrane; nuclear matrix; nuclear membrane; nucleoplasm. Molecular Function: phospholipase binding; structural molecule activity. Disease: Leukodystrophy, Demyelinating, Adult-onset, Autosomal Dominant

0.1 mg

305 USD