antibody

Goat anti-SLC46A1/PCFT (aa233-247) Antibody

MBS422880

0.1 mg

305 USD

polyclonal

goat

nonconjugated

western blot, enzyme immunoassay

Antibody

Goat anti-SLC46A1/PCFT (aa233-247) Antibody

[SLC46A1/PCFT]

[Goat Anti-SLC46A1 / PCFT Antibody; SLC46A1; solute carrier family 46 (folate transporter), member 1; FLJ39875; HCP1; MGC9564; PCFT; G21; PCFT/HCP1; heme carrier protein 1; proton-coupled folate transporter; solute carrier family 46 member 1; SLC46A1 antibody; solute carrier family 46 (folate transporter); member 1 antibody; FLJ39875 antibody; HCP1 antibody; MGC9564 antibody; PCFT antibody; G21 antibody; PCFT/HCP1 antibody; heme carrier protein 1 antibody; solute carrier family 46 member 1 antibody; SLC46A1 / PCFT (aa233-247)]

[proton-coupled folate transporter isoform 1; Proton-coupled folate transporter; proton-coupled folate transporter; solute carrier family 46 member 1; G21; Heme carrier protein 1; PCFT/HCP1; Solute carrier family 46 member 1]

[SLC46A1]

[PCFT]

[SLC46A1; SLC46A1; G21; HCP1; PCFT; HCP1; PCFT]

PCFT_HUMAN

Polyclonal

Goat

Tested: Human; Expected from sequence similarity: Human

459

LKEPKSTRLFTFRH

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB)

Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 50kDa band observed in Human Kidney lysates (calculated MW of 49.8kDa according to NP_542400.2). Recommended concentration: 0.3-1ug/ml.

Western Blot (WB)

Immunogen: Peptide with sequence C-LKEPKSTRLFTFRH, from the internal region of the protein sequence according to NP_542400.2.

31543204

NP_542400.2

NM_080669.5

Iron Uptake And Transport Pathway (1269948); Metabolism Pathway (1269956); Metabolism Of Folate And Pterines Pathway (1270156); Metabolism Of Vitamins And Cofactors Pathway (1270144); Metabolism Of Water-soluble Vitamins And Cofactors Pathway (1270145); Mineral Absorption Pathway (212237); Mineral Absorption Pathway (212220); Transmembrane Transport Of Small Molecules Pathway (1269903); Vitamin Digestion And Absorption Pathway (199556); Vitamin Digestion And Absorption Pathway (199538)

This gene encodes a transmembrane proton-coupled folate transporter protein that facilitates the movement of folate and antifolate substrates across cell membranes, optimally in acidic pH environments. This protein is also expressed in the brain and choroid plexus where it transports folates into the central nervous system. This protein further functions as a heme transporter in duodenal enterocytes, and potentially in other tissues like liver and kidney. Its localization to the apical membrane or cytoplasm of intestinal cells is modulated by dietary iron levels. Mutations in this gene are associated with autosomal recessive hereditary folate malabsorption disease. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2013]

SLC46A1: Has been shown to act both as an intestinal proton- coupled high-affinity folate transporter and as an intestinal heme transporter which mediates heme uptake from the gut lumen into duodenal epithelial cells. The iron is then released from heme and may be transported into the bloodstream. Dietary heme iron is an important nutritional source of iron. Shows a higher affinity for folate than heme. Defects in SLC46A1 are the cause of hereditary folate malabsorption (HFM). HFM is a rare autosomal recessive disorder characterized by impaired intestinal folate absorption with folate deficiency resulting in anemia, hypoimmunoglobulinemia with recurrent infections, and recurrent or chronic diarrhea. In many patients, neurological abnormalities such as seizures or mental retardation become apparent during early childhood, attributed to impaired transport of folates into the central nervous system. When diagnosed early, the disorder can be treated by administration of folate. If untreated, it can be fatal and, if treatment is delayed, the neurological defects can become permanent. Belongs to the major facilitator superfamily. SLC46A family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, multi-pass; Transporter, iron; Membrane protein, integral; Transporter; Transporter, ion channel. Chromosomal Location of Human Ortholog: 17q11.2. Cellular Component: apical plasma membrane; brush border membrane; cell surface; cytoplasm; integral to membrane; plasma membrane. Molecular Function: folic acid binding; folic acid transporter activity; heme transporter activity; hydrogen ion transmembrane transporter activity; methotrexate transporter activity. Biological Process: cellular iron ion homeostasis; folic acid metabolic process; folic acid transport; heme transport; methotrexate transport. Disease: Folate Malabsorption, Hereditary

0.1 mg

305 USD