Goat anti-CB1 (aa44-58) Antibody | MyBioSource MBS422809 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-CB1 (aa44-58) Antibody

catalog :

MBS422809

quantity :

0.1 mg

price :

305 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, cow

application :

western blot, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

(0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

product information

catalog number :

MBS422809

products type :

Antibody

products full name :

Goat anti-CB1 (aa44-58) Antibody

products short name :

[CB1]

products name syn :

[CNR1, cannabinoid receptor 1 (brain), CANN6, CB-R, CB1, CB1A, CB1K5, CB1R, CNR, cannabinoid receptor 1, central cannabinoid receptor]

other names :

[cannabinoid receptor 1 isoform a; Cannabinoid receptor 1; cannabinoid receptor 1; cannabinoid receptor 1 (brain); CANN6]

products gene name :

[CNR1]

products gene name syn :

[CB1]

other gene names :

[CNR1; CNR1; CB1; CNR; CB-R; CB1A; CB1R; CANN6; CB1K5; CNR; CB-R; CB1]

uniprot entry name :

CNR1_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

sequence length :

472

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 70+55kDa bands observed in Rat Brain lysates (calculated MW of 52.9kDa according to Human NP_057167.2 and 52.8kDa according to Rat NP_036916.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Porcella et al, Eur J Neurosci.2000 Mar;12(3): 1123-7. PMID: 10762343). Recommended concentration: 1-3ug/ml.

image1 heading :

Western Blot (WB)

other info1 :

Immunogen: Peptide with sequence C-NITEFYNKSLSSFKE, from the internal region (near N Terminus) of the protein sequence according to NP_057167.2; NP_149421.2.

other info2 :

Note: This antibody is expected to recognize both reported isoforms (NP_057167.2; NP_149421.2). Reported variants represent identical protein: NP_001153731.1, NP_001153732.1, NP_057167.2, NP_001153730.1, NP_001153698.1

ncbi gi num :

38683844

ncbi acc num :

NP_057167.2

ncbi gb acc num :

NM_016083.4

ncbi pathways :

BDNF Signaling Pathway (712093); Class A/1 (Rhodopsin-like Receptors) Pathway (1269545); G Alpha (i) Signalling Events Pathway (1269576); GPCR Downstream Signaling Pathway (1269574); GPCR Ligand Binding Pathway (1269544); GPCRs, Class A Rhodopsin-like Pathway (198886); N-cadherin Signaling Events Pathway (137919); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462); Rap1 Signaling Pathway (868086)

ncbi summary :

This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene. [provided by RefSeq, May 2009]

uniprot summary :

CNR1: Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding. Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1. Chromosomal Location of Human Ortholog: 6q14-q15. Cellular Component: axon; growth cone; integral to plasma membrane; intracellular membrane-bound organelle; lipid raft; plasma membrane. Molecular Function: cannabinoid receptor activity; drug binding. Biological Process: aging; axonal fasciculation; G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein signaling, coupled to cyclic nucleotide second messenger; glucose homeostasis; maternal process involved in pregnancy; memory; negative regulation of action potential; negative regulation of blood pressure; negative regulation of dopamine secretion; negative regulation of fatty acid beta-oxidation; negative regulation of mast cell activation; negative regulation of nitric-oxide synthase activity; positive regulation of acute inflammatory response to antigenic stimulus; positive regulation of apoptosis; positive regulation of blood pressure; positive regulation of fever; regulation of insulin secretion; regulation of synaptic transmission, GABAergic; regulation of synaptic transmission, glutamatergic; response to cocaine; response to ethanol; response to lipopolysaccharide; response to morphine; response to nicotine; response to nutrient; sensory perception of pain; spermatogenesis

size1 :

0.1 mg

price1 :

305 USD

more info or order :

MyBioSource product webpage