antibody

Goat anti-Tfb2m (mouse) Antibody

MBS422745

0.1 mg

300 USD

polyclonal

goat

nonconjugated

mouse, rat

western blot, enzyme immunoassay

Antibody

Goat anti-Tfb2m (mouse) Antibody

Tfb2m

Tfb2m; transcription factor B2, mitochondrial; Hkp1; OTTMUSP00000025719; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 2; dimethyladenosine transferase 2, mitochondrial; house-keeping protein 1; mTFB2M; mitochondrial 12S rRNA dimethylas; Tfb2m antibody; transcription factor B2; mitochondrial antibody; Hkp1 antibody; OTTMUSP00000025719 antibody; S-adenosylmethionine-6-N'; N'-adenosyl(rRNA) dimethyltransferase 2 antibody; dimethyladenosine transferase 2; mitochondrial antibody; house-keeping protein 1 antibody; mTFB2M antibody; mitochondrial 12S rRNA dimethylase 2 antibody; mitochondrial transcription factor B2 antibody; mtTFB2 antibody; Tfb2m (mouse)

dimethyladenosine transferase 2, mitochondrial; Dimethyladenosine transferase 2, mitochondrial; dimethyladenosine transferase 2, mitochondrial; transcription factor B2, mitochondrial; Mitochondrial 12S rRNA dimethylase 2; Mitochondrial transcription factor B2; mTFB2M; mtTFB2; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase 2

Tfb2m

Tfb2m; Tfb2m; Hkp1; mTFB2M; mtTFB2

TFB2M_MOUSE

Polyclonal

Goat

Tested: Mouse, Rat; Expected from sequence similarity: Mouse, Rat

396

RNLVRDLLEHQNPS

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB)

Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Approx 37kDa band observed in Mouse and Rat Lung lysates (calculated MW of 45.9kDa according to NP_032275.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-2ug/ml.

Immunogen: Peptide with sequence C-RNLVRDLLEHQNPS, from the internal region of the protein sequence according to NP_032275.2. Epitope: Internal region

84490373

NP_032275.2

NM_008249.4

45,864 Da

Gene Expression Pathway (1367365); Mitochondrial Gene Expression Pathway (198322); Mitochondrial Biogenesis Pathway (1367351); Mitochondrial Transcription Initiation Pathway (1367404); Organelle Biogenesis And Maintenance Pathway (1367350); RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway (1367390); Transcription From Mitochondrial Promoters Pathway (1367403); Transcriptional Activation Of Mitochondrial Biogenesis Pathway (1367353)

TFB2M: S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. Also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. Stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity. Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. KsgA subfamily. Protein type: Methyltransferase; Transcription, coactivator/corepressor; EC 2.1.1.-. Cellular Component: cell junction; mitochondrial matrix; mitochondrion. Molecular Function: 1-phenanthrol methyltransferase activity; 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity; arginine N-methyltransferase activity; C-methyltransferase activity; C-terminal protein carboxyl methyltransferase activity; cobalt-precorrin-3 C17-methyltransferase activity; cobalt-precorrin-5B C1-methyltransferase activity; cobalt-precorrin-6B C5-methyltransferase activity; cobalt-precorrin-7 C15-methyltransferase activity; demethylmenaquinone methyltransferase activity; dimethylamine methyltransferase activity; DNA-methyltransferase activity; hydroxyneurosporene-O-methyltransferase activity; lysine N-methyltransferase activity; methanol-specific methylcobalamin:coenzyme M methyltransferase activity; methylamine-specific methylcobalamin:coenzyme M methyltransferase activity; methylarsonite methyltransferase activity; methyltransferase activity; monomethylamine methyltransferase activity; mRNA methyltransferase activity; N-methyltransferase activity; O-methyltransferase activity; protein methyltransferase activity; protein-arginine N-methyltransferase activity; protein-arginine N5-methyltransferase activity; protein-leucine O-methyltransferase activity; protein-lysine N-methyltransferase activity; RNA binding; RNA methyltransferase activity; rRNA (adenine) methyltransferase activity; rRNA (adenine-N6,N6-)-dimethyltransferase activity; rRNA (adenine-N6-)-methyltransferase activity; rRNA (cytosine) methyltransferase activity; rRNA (cytosine-C5-967)-methyltransferase activity; rRNA (guanine) methyltransferase activity; rRNA (uridine) methyltransferase activity; rRNA (uridine-2'-O-)-methyltransferase activity; rRNA methyltransferase activity; S-methyltransferase activity; selenocysteine methyltransferase activity; transcription cofactor activity; transferase activity; trimethylamine methyltransferase activity; tRNA (adenine)-methyltransferase activity; tRNA (adenine-57, 58-N(1)-) methyltransferase activity; tRNA (adenine-N1-)-methyltransferase activity; tRNA (cytosine)-methyltransferase activity; tRNA (cytosine-5-)-methyltransferase activity; tRNA (guanine) methyltransferase activity; tRNA (guanine-N1-)-methyltransferase activity; tRNA (guanine-N2-)-methyltransferase activity; tRNA (uracil) methyltransferase activity; tRNA methyltransferase activity. Biological Process: methylation; positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent; rRNA methylation; rRNA processing; transcription from mitochondrial promoter; transcription initiation from mitochondrial promoter; transcription, DNA-dependent

0.1 mg

300 USD