antibody

Goat anti-Orexin receptor 1 Antibody

MBS422563

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog

western blot, enzyme immunoassay

Antibody

Goat anti-Orexin receptor 1 Antibody

Orexin receptor 1

Goat Anti-OX1R and OX2R Antibody; HCRTR1; OX1R; hypocretin (orexin) receptor 1; orexin receptor; orexin receptor 1; orexin receptor-1; hypocretin receptor 1; hypocretin receptor-1; HCRTR1 antibody; OX1R antibody; hypocretin (orexin) receptor 1 antibody; orexin receptor antibody; orexin receptor 1 antibody; orexin receptor-1 antibody; hypocretin receptor 1 antibody; hypocretin receptor-1 antibody; Orexin receptor 1; Orexin receptor 1

orexin receptor 1; Orexin receptor type 1; orexin receptor type 1; hypocretin receptor 1; Hypocretin receptor type 1

HCRTR1

HCRTR1; HCRTR1; OX1R; Ox-1-R; Ox1-R; Ox1R

OX1R_HUMAN

Polyclonal

Goat

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Sheep

425

RNWKRPSDQLGD

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB)

Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx 48+35kDa band observed in Human Brain lysates (calculated MW of 47.5kDa according to NP_001516.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Silveyra et al, Am J Physiol Endocrinol Metab.2007 Oct;293(4): E977-85; PMID: 17638707). Recommended concentration: 0.1-0.3ug/ml.

Immunogen: Peptide with sequence C-RNWKRPSDQLGD, from the internal region of the protein sequence according to NP_001516.1. Epitope: Internal region

4557637

NP_001516.1

NM_001525.1

47,536 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (1269545); G Alpha (q) Signalling Events Pathway (1269578); GPCR Downstream Signaling Pathway (1269574); GPCR Ligand Binding Pathway (1269544); GPCRs, Class A Rhodopsin-like Pathway (198886); Gastrin-CREB Signalling Pathway Via PKC And MAPK (1269592); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462); Orexin And Neuropeptides FF And QRFP Bind To Their Respective Receptors Pathway (1269550); Peptide Ligand-binding Receptors Pathway (1269546)

The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein selectively binds the hypothalamic neuropeptide orexin A. A related gene (HCRTR2) encodes a G-protein coupled receptor that binds orexin A and orexin B. [provided by RefSeq, Jan 2009]

HCRTR1: Moderately selective excitatory receptor for orexin-A and, with a lower affinity, for orexin-B neuropeptide. Seems to be exclusively coupled to the G(q) subclass of heteromeric G proteins, which activates the phospholipase C mediated signaling cascade. Belongs to the G-protein coupled receptor 1 family. Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 1p33. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: G-protein coupled receptor activity; neuropeptide receptor activity; orexin receptor activity; peptide binding; peptide hormone binding. Biological Process: cellular response to hormone stimulus; feeding behavior; neuropeptide signaling pathway; regulation of circadian sleep/wake cycle, sleep; synaptic transmission

0.1 mg

300 USD