antibody

Goat anti-DCTN3 Antibody

MBS422499

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog, cow

western blot, enzyme immunoassay

Antibody

Goat anti-DCTN3 Antibody

DCTN3

DCTN3; dynactin 3 (p22); DCTN-22; DCTN22; MGC111190; dynactin 3; dynactin 3, isoform 1; dynactin light chain; DCTN22 antibody; DCTN-22 antibody; dynactin 3 antibody; dynactin 3 (p22) antibody; dynactin 3; isoform 1 antibody; dynactin light chain antibody; MGC111190 antibody; DCTN3 antibody

dynactin subunit 3 isoform 1; Dynactin subunit 3; dynactin subunit 3; dynactin subunit 3; Dynactin complex subunit 22 kDa subunit; p22

DCTN3

DCTN3; DCTN3; DCTN22; DCTN-22; p22

DCTN3_HUMAN

Polyclonal

Goat

Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

186

QVALGNISSKRE

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB)

Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: Approx 20kDa band observed in Human Kidney, Skeletal Muscle and Pancreas lysates (calculated MW of 19.5kDa according to NP_077324.1). In addition, a 25kDa band is observed in both Mouse and Rat Skeletal Muscle lysates accompanied by an 18kDa band in Mouse Skeletal Msuscle. In Mouse the protein is known as p24. The 18kDa band could reprsent a smaller isoform. Recommended concentration: 0. 03-0.1ug/ml.

Immunogen: Peptide with sequence C-QVALGNISSKRE, from the internal region of the protein sequence according to NP_009165.1; NP_077324.1. Epitope: Internal region

Note: This antibody is expected to recognize both reported isoforms (NP_009165.1; NP_077324.1).

6005745

NP_009165.1

NM_007234.4

18,001 Da

AURKA Activation By TPX2 Pathway (1383018); Adaptive Immune System Pathway (1269171); Anchoring Of The Basal Body To The Plasma Membrane Pathway (1268847); Asparagine N-linked Glycosylation Pathway (1268714); Assembly Of The Primary Cilium Pathway (1268846); COPI-independent Golgi-to-ER Retrograde Traffic Pathway (1383043); COPI-mediated Anterograde Transport Pathway (1339109); Cell Cycle Pathway (1269741); Cell Cycle, Mitotic Pathway (1269763); Centrosome Maturation Pathway (1269805)

This gene encodes the smallest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, cytokinesis, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like most other dynactin subunits, exists only as a part of the dynactin complex. It is primarily an alpha-helical protein with very little coiled coil, and binds directly to the largest subunit (p150) of dynactin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

dynactin 3: Together with dynein may be involved in spindle assembly and cytokinesis. Belongs to the dynactin subunit 3 family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Microtubule-binding; Motility/polarity/chemotaxis; Motor. Chromosomal Location of Human Ortholog: 9p13. Cellular Component: centrosome; cleavage furrow; cytosol; dynactin complex; midbody; perinuclear region of cytoplasm; spindle. Molecular Function: protein binding; structural molecule activity. Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; cytokinesis; ER to Golgi vesicle-mediated transport; G2/M transition of mitotic cell cycle; microtubule-based process; mitosis

0.1 mg

300 USD