Goat anti-MYO5A Antibody | MyBioSource MBS422444 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-MYO5A Antibody

catalog :

MBS422444

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, cow

application :

western blot, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS422444

products type :

Antibody

products full name :

Goat anti-MYO5A Antibody

products short name :

MYO5A

products name syn :

MYO5A; myosin VA (heavy chain 12, myoxin); GS1; MYH12; MYO5; MYR12; dilute; myosin V; myosin VA; myosin, heavy polypeptide kinase; myoxin; dilute antibody; GS1 antibody; MYH12 antibody; MYO5 antibody; myosin V antibody; myosin VA antibody; myosin VA (heavy chain 12; myoxin) antibody; myosin; heavy polypeptide kinase antibody; myoxin antibody; MYR12 antibody; MYO5A antibody

other names :

unconventional myosin-Va isoform 1; Unconventional myosin-Va; unconventional myosin-Va; myosin VA; Dilute myosin heavy chain, non-muscle; Myosin heavy chain 12; Myosin-12; Myoxin

products gene name :

MYO5A

other gene names :

MYO5A; MYO5A; GS1; MYO5; MYH12; MYR12; MYH12

uniprot entry name :

MYO5A_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Cow

sequence length :

1855

sequence :

ETKQLELDLN

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx 200kDa band observed in lysates of cell line Jurkat (calculated MW of 215kDa according to NP_000250.3). Recommended concentration: 1-3ug/ml. Immunofluorescence: Transverse sections of Mouse Skeletal Muscle shows staining of the fibre membrane and neuromuscular junction. The observed pattern corresponds to earlier findings in literature with different antibodies (Röder et al, Proc Natl Acad Sci U S A.2010 Feb 2;107(5): 2031-6; PMID: 20133847). Recommended concentration: 1-3ug/ml.

other info1 :

Immunogen: Peptide with sequence C-ETKQLELDLN, from the internal region of the protein sequence according to NP_000250.3; NP_001135967.1. Epitope: Internal region

other info2 :

Note: This antibody is expected to recognize both reported isoforms (NP_000250.3; NP_001135967.1).

ncbi gi num :

215982791

ncbi acc num :

NP_000250.3

ncbi gb acc num :

NM_000259.3

ncbi mol weight :

218,608 Da

ncbi pathways :

Fcgamma Receptor (FCGR) Dependent Phagocytosis Pathway (1269279); Immune System Pathway (1269170); Innate Immune System Pathway (1269203); Insulin Processing Pathway (1268747); Membrane Trafficking Pathway (1269877); Metabolism Of Proteins Pathway (1268677); Peptide Hormone Metabolism Pathway (1268746); Regulation Of Actin Dynamics For Phagocytic Cup Formation Pathway (1269281); Translocation Of GLUT4 To The Plasma Membrane Pathway (1269896); Vesicle-mediated Transport Pathway (1269876)

ncbi summary :

This gene is one of three myosin V heavy-chain genes, belonging to the myosin gene superfamily. Myosin V is a class of actin-based motor proteins involved in cytoplasmic vesicle transport and anchorage, spindle-pole alignment and mRNA translocation. The protein encoded by this gene is abundant in melanocytes and nerve cells. Mutations in this gene cause Griscelli syndrome type-1 (GS1), Griscelli syndrome type-3 (GS3) and neuroectodermal melanolysosomal disease, or Elejalde disease. Multiple alternatively spliced transcript variants encoding different isoforms have been reported, but the full-length nature of some variants has not been determined. [provided by RefSeq, Dec 2008]

uniprot summary :

MYO5A: Processive actin-based motor that can move in large steps approximating the 36-nm pseudo-repeat of the actin filament. Involved in melanosome transport. May also be required for some polarization process involved in dendrite formation. May be a homodimer, which associates with multiple calmodulin or myosin light chains. Binds MLPH and MYRIP. Interacts with RIPL2, the interaction is required for its role in dendrite formation. Detected in melanocytes. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Motor. Chromosomal Location of Human Ortholog: 15q21. Cellular Component: actin filament; actomyosin; cell soma; cytoplasm; cytosol; early endosome; endoplasmic reticulum; filopodium tip; Golgi apparatus; growth cone; intermediate filament; late endosome; lysosome; melanosome; membrane; myosin complex; neuron projection; peroxisome; photoreceptor outer segment; recycling endosome; ruffle; vesicle. Molecular Function: actin binding; ATP binding; calcium ion binding; calmodulin binding; microfilament motor activity; Rab GTPase binding. Biological Process: actin filament-based movement; cellular response to insulin stimulus; ER localization; exocytosis; insulin secretion; locomotion during locomotory behavior; long-chain fatty acid biosynthetic process; melanin biosynthetic process; melanocyte differentiation; melanosome transport; myelination; odontogenesis; post-Golgi vesicle-mediated transport; regulation of inositol-1,4,5-triphosphate receptor activity; secretory granule localization; synapse organization and biogenesis; synaptic transmission; transport; vesicle transport along actin filament; vesicle-mediated transport; visual perception. Disease: Griscelli Syndrome, Type 1

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage