antibody

Goat anti-Glutamate Dehydrogenase Antibody

MBS422307

0.1 mg

290 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog, cow

western blot, immunohistochemistry, immunocytochemistry, enzyme immunoassay

Antibody

Goat anti-Glutamate Dehydrogenase Antibody

Glutamate Dehydrogenase

GDH; GDH1; GLUD; glutamate dehydrogenase 1; MGC132003; GLUD1; GLUD1; glutamate dehydrogenase 1; GDH; GDH1; GLUD; MGC132003; glutamate dehydrogenase (NAD(P)+); GLUD2; glutamate dehydrogenase 2; GDH2; GLUDP1; glutamate dehydrogenase pseudogene 1

glutamate dehydrogenase 1, mitochondrial; Glutamate dehydrogenase 1, mitochondrial; glutamate dehydrogenase 1, mitochondrial; GDH 1; glutamate dehydrogenase (NAD(P)+); glutamate dehydrogenase 1

GLUD1/GLUD2

GLUD1; GLUD1; GDH; GDH1; GLUD; GLUD; GDH 1

DHE3_HUMAN

Polyclonal

Goat

Human, Mouse, Rat, Dog, Cow

558

ESEEQKRNRVRGILR

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Immunohistochemistry: In paraffin embedded Human Cerebral Cortex shows mitochondrial staining in neuronal cell bodies and axons. Recommended concentration, 3-5ug/ml. Western Blot: Approx 55kDa band observed in Human Liver and in Mouse Liver lysates (calculated MW of 61.4kDa according to Human NP_005262.1 and NP_036216.2 and of 61.3 according to Mouse NP_032159.1. Recommended concentration: 0.3-1ug/ml. Peptide ELISA: antibody detection limit dilution 1:32000. Immunofluorescence: Customer found particulate cytoplasm staining in HeLa

Epitope: Internal Region. Immunogen: Peptide with sequence C-ESEEQKRNRVRGILR, from the internal region of the protein sequence according to NP_005262.1; NP_036216.2. Corresponding Immunizing Peptide: Catalog #MBS427215

Important Note: This antibody is expected to recognize both reported isoforms (NP_005262.1; NP_036216.2).

4885281

NP_005262.1

NM_005271.3

46,575 Da

Alanine, Aspartate And Glutamate Metabolism Pathway 101142!!Alanine, Aspartate And Glutamate Metabolism Pathway 100063!!Amino Acid Synthesis And Interconversion (transamination) Pathway 106173!!Arginine And Proline Metabolism Pathway 82957!!Arginine And Proline Metabolism Pathway 323!!D-Glutamine And D-glutamate Metabolism Pathway 82971!!D-Glutamine And D-glutamate Metabolism Pathway 340!!Metabolic Pathways 132956!!Metabolism Pathway 477135!!Metabolism Of Amino Acids And Derivatives Pathway 106169

This gene encodes glutamate dehydrogenase protein; a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid induced insulin secretion and activating mutations in this gene are a common cause of congenital hyperinsulinism. This enzyme is allosterically activated by ADP and inhibited by GTP and ATP. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Multiple pseudogenes of this gene are present in humans.[provided by RefSeq, Sep 2009]

0.1 mg

290 USD