Goat anti-SRGAP2 Antibody | MyBioSource MBS422275 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-SRGAP2 Antibody

catalog :

MBS422275

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, cow

application :

immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS422275

products type :

Antibody

products full name :

Goat anti-SRGAP2 Antibody

products short name :

SRGAP2

products name syn :

SRGAP2; SLIT-ROBO Rho GTPase activating protein 2; FNBP2; KIAA0456; srGAP3; formin binding protein 2; SRGAP2 antibody; FNBP2 antibody; formin binding protein 2 antibody; KIAA0456 antibody; SLIT-ROBO Rho GTPase activating protein 2 antibody; srGAP3 antibody

other names :

SLIT-ROBO Rho GTPase-activating protein 2 isoform a; SLIT-ROBO Rho GTPase-activating protein 2; SLIT-ROBO Rho GTPase-activating protein 2; SLIT-ROBO Rho GTPase activating protein 2; Formin-binding protein 2; Rho GTPase-activating protein 34

products gene name :

SRGAP2

other gene names :

SRGAP2; SRGAP2; FNBP2; SRGAP3; SRGAP2A; ARHGAP34; ARHGAP34; FNBP2; KIAA0456; SRGAP2A; srGAP2

uniprot entry name :

SRGP2_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

sequence length :

1071

sequence :

KQEDRQTPRSPDST

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Immunofluorescence (IF)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx 124kDa band observed in lysates of cell line HeLa and in rat primary culture cortical neurons at Day 4 (calculated MW of 121kDa according to human NP_056141.2 but the rat protein sequence is stlll provisional). Recommended concentration: 0.1-0.3ug/ml. Immunofluorescence: Transfected HEK293 transiently expressing GFP-tagged Human srGAP2 with the primary antibody stained in the red channel (Confocal IF).

other info1 :

Immunogen: Peptide with sequence C-KQEDRQTPRSPDST, from the internal region of the protein sequence according to NP_056141.2. Epitope: Internal region

other info2 :

Note: This antibody is expected to recognize isoform a (NP_056141.2).

ncbi gi num :

112363100

ncbi acc num :

NP_056141.2

ncbi gb acc num :

NM_015326.4

ncbi mol weight :

120,881 Da

ncbi pathways :

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (1270303); Developmental Biology Pathway (1270302); Inactivation Of Cdc42 And Rac Pathway (1270320); RHO GTPase Effectors Pathway (1269509); RHO GTPases Activate Formins Pathway (1269519); Rho GTPase Cycle Pathway (1269508); Signal Transduction Pathway (1269379); Signaling By Rho GTPases Pathway (1269507)

ncbi summary :

This locus encodes a member of the SLIT-ROBO Rho GTPase activating protein family. The encoded protein stimulates GTPase activity of Rac1, and plays a role in cortical neuron development. This locus has several paralogs on human chromosome 1 resulting from segmental duplication. While this locus itself is conserved among various species, the paralogs are found only in the genus Homo, and not in the genomes of non-human great apes. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Jul 2014]

uniprot summary :

SRGAP2: RAC1 GTPase activating protein (GAP) that binds and deforms membranes, and regulates actin dynamics to regulate cell migration and differentiation. Plays an important role in different aspects of neuronal morphogenesis and migration mainly during development of the cerebral cortex. This includes the biogenesis of neurites, where it is required for both axons and dendrites outgrowth, and the maturation of the dendritic spines. Also stimulates the branching of the leading process and negatively regulates neuron radial migration in the cerebral cortex. Its interaction and inhibition by SRGAP2C reduces the rate of spine maturation, alters dendritic spine morphology and density and indirectly increases neuronal migration. It may have implications for cognition, learning and memory. In non-neuronal cells, it may also play a role in cell migration by regulating the formation of lamellipodia and filopodia. A chromosomal aberration disrupting SRGAP2 has been found in a patient with early infantile epileptic encephalopathy. Balanced translocation t(1;9)(q32;q13). Protein type: GAPs; Mitochondrial; GAPs, Rac/Rho; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 1q32.1. Cellular Component: cell junction; cytoplasm; cytosol; lamellipodium; nucleus; phagocytic vesicle; plasma membrane; postsynaptic density; postsynaptic membrane. Molecular Function: GTPase activator activity; protein binding; protein homodimerization activity; Rac GTPase binding. Biological Process: actin filament severing; cell proliferation; extension of a leading process involved in cell locomotion in cerebral cortex radial glia guided migration; filopodium formation; neurite morphogenesis; positive regulation of GTPase activity; regulation of small GTPase mediated signal transduction; signal transduction

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage