Goat anti-Trem2 (mouse) Antibody | MyBioSource MBS422239 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-Trem2 (mouse) Antibody

catalog :

MBS422239

quantity :

0.1 mg

price :

305 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

mouse

application :

western blot, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

(0.3ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

image 2 :

Immunofluorescence analysis of paraformaldehyde fixed 3T3-L1 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

product information

catalog number :

MBS422239

products type :

Antibody

products full name :

Goat anti-Trem2 (mouse) Antibody

products short name :

[Trem2]

products name syn :

[Trem2, triggering receptor expressed on myeloid cells 2, Trem2a, Trem2b, Trem2c, triggering receptor expressed on myeloid cells 2a, triggering receptor expressed on myeloid cells 2b, triggering receptor expressed on myeloid cells 2c]

other names :

[triggering receptor expressed on myeloid cells 2 isoform 1; Triggering receptor expressed on myeloid cells 2; triggering receptor expressed on myeloid cells 2; triggering receptor expressed on myeloid cells 2; Triggering receptor expressed on monocytes 2]

products gene name :

[Trem2]

other gene names :

[Trem2; Trem2; TREM-2; Trem2a; Trem2b; Trem2c; Trem2a; Trem2b; Trem2c; TREM-2]

uniprot entry name :

TREM2_MOUSE

clonality :

polyclonal

host :

goat

reactivity :

Tested: Mouse. Expected from sequence similarity: Mouse, Rat

sequence length :

227

sequence :

QVEHSTSRNQET

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

storage stability :

Aliquot and store at -20°C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB)

app notes :

. Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Preliminary experiments showed a band at approx 26 kDa in Mouse and Rat Adrenal gland lysates after 1 ug/ml antibody staining (calculated MW of 24.5 kDa according to Mouse NP_112544.1 and 24.9 according to Rat NP_001100354.1). Primary incubation 1 hour at room temperature. Immunofluorescence: Strong expression of the protein seen in the Plasma Membrane of NIH3T3-L1 (adipocyte;like) cells. Recommended concentration: 10 ug/ml.

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence

other info1 :

Immunogen: Peptide with sequence C-QVEHSTSRNQET, from the internal region of the protein sequence according to NP_112544.1.

ncbi gi num :

13994125

ncbi acc num :

NP_112544.1

ncbi gb acc num :

NM_031254.3

ncbi pathways :

Adaptive Immune System Pathway (1367473); Axon Guidance Pathway (1366709); DAP12 Interactions Pathway (1367572); DAP12 Signaling Pathway (1367573); Developmental Biology Pathway (1366708); Immune System Pathway (1367472); Immunoregulatory Interactions Between A Lymphoid And A Non-Lymphoid Cell Pathway (1367500); Innate Immune System Pathway (1367502); Osteoclast Differentiation Pathway (193149); Osteoclast Differentiation Pathway (193096)

ncbi summary :

The protein encoded by this gene is part of the immunoglobulin and lectin-like superfamily and functions as part of the innate immune system. This gene forms part of a cluster of genes on mouse chromosome 17 thought to be involved in innate immunity. This protein associates with the adaptor protein Dap-12 and recruits several factors, such as kinases and phospholipase C-gamma, to form a receptor signaling complex that activates myeloid cells, including dendritic cells and microglia. In humans homozygous loss-of-function mutations in this gene cause Nasu-Hakola disease and mutations in this gene may be risk factors to the development of Alzheimer's disease. In mouse mutations of this gene serve as a pathophysiological model for polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (Nasu-Hakola disease) and for inflammatory bowel disease. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]

uniprot summary :

TREM2: May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL); also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Cellular Component: extracellular region; integral to membrane; intracellular membrane-bound organelle; membrane; plasma membrane. Molecular Function: lipopolysaccharide binding; peptidoglycan binding; protein binding; transmembrane receptor activity. Biological Process: detection of lipopolysaccharide; detection of peptidoglycan; innate immune response; lipopolysaccharide-mediated signaling pathway; phagocytosis, engulfment; positive regulation of antigen processing and presentation of peptide antigen via MHC class II; positive regulation of calcium-mediated signaling; positive regulation of peptidyl-tyrosine phosphorylation

size1 :

0.1 mg

price1 :

305 USD

more info or order :

MyBioSource product webpage