antibody

Goat anti-Calnexin Antibody

MBS422230

0.1 mg

290 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog, cow

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-Calnexin Antibody

Calnexin

CANX; calnexin; CNX; FLJ26570; IP90; P90; major histocompatibility complex class I antigen-binding protein p88; CANX; calnexin; CNX; FLJ26570; IP90; P90; major histocompatibility complex class I antigen-binding protein p88

calnexin; Calnexin; calnexin; major histocompatibility complex class I antigen-binding protein p88; calnexin; IP90; Major histocompatibility complex class I antigen-binding protein p88; p90

CANX

CANX; CANX; CNX; P90; IP90

CALX_HUMAN

Polyclonal

Goat

Human, Mouse, Rat, Dog, Pig, Cow

592

SKTPELNLDQFHDKT

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

Immunohistochemistry: In paraffin embedded Human Kidney shows textured staining in the cytoiplasm of DCT cells,. Recommended concentration, 3-6ug/ml. Western Blot: Approx. 100kDa band observed in Mouse Heart, Human Brain (Cerebellum), NIH3T3 and HeLa lysates (calculated MW of 67.6kDa according to Human NP_001737.1 and 67.2kDa according to Mouse NP_001103969.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Oliver et al., J Biol Chem. 1996 Jun 7;271(23):13691-6., PMID: 8662691). Recommended concentration: 0.3-1ug/ml. Peptide ELISA: antibody detection limit dilution 1:32000. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant Calnexin captured by a rabbit antibody. Recommended reporter concentration: 1-2ug/ml

Epitope: Internal Region (Near N Terminus). Immunogen: Peptide with sequence C-SKTPELNLDQFHDKT, from the internal region (near N Terminus) of the protein sequence according to NP_001737.1. Corresponding Immunizing Peptide: Catalog #MBS426398

Important Note: Reported variants represent identical protein (NP_001019820.1, NP_001737.1).

10716563

NP_001737.1

NM_001746.3

55,598 Da

Adaptive Immune System Pathway 366160!!Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway 366163!!Antigen Processing And Presentation Pathway 83074!!Antigen Processing And Presentation Pathway 485!!Asparagine N-linked Glycosylation Pathway 161013!!Assembly Of Viral Components At The Budding Site Pathway 106084!!Calnexin/calreticulin Cycle Pathway 187218!!Class I MHC Mediated Antigen Processing Presentation Pathway 366161!!Disease Pathway 530764!!HTLV-I Infection Pathway 373901

This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]

0.1 mg

290 USD