catalog number :
MBS422194
products full name :
Goat anti-CSX1 / NKX2-5 Antibody
products short name :
CSX1/NKX2-5
products name syn :
NKX2-5; NK2 transcription factor related; locus 5 (Drosophila); CHNG5; CSX; CSX1; NKX2.5; NKX2E; NKX4-1; NK2 transcription factor homolog E; NK2 transcription factor related; locus 5; cardiac-specific homeo box; tinman paralog; NKX2-5; NK2 transcription factor related, locus 5 (Drosophila); CHNG5; CSX; CSX1; NKX2.5; NKX2E; NKX4-1; NK2 transcription factor homolog E; NK2 transcription factor related, locus 5; cardiac-specific homeo box; tinman paralog
other names :
homeobox protein Nkx-2.5 isoform 1; Homeobox protein Nkx-2.5; homeobox protein Nkx-2.5; tinman paralog; homeobox protein CSX; cardiac-specific homeobox 1; homeobox protein NK-2 homolog E; NK2 transcription factor related, locus 5; NK2 homeobox 5; Cardiac-specific homeobox; Homeobox protein CSX; Homeobox protein NK-2 homolog E
products gene name :
NKX2-5
other gene names :
NKX2-5; NKX2-5; CSX; CSX1; VSD3; CHNG5; HLHS2; NKX2E; NKX2.5; NKX4-1; CSX; NKX2.5; NKX2E
uniprot entry name :
NKX25_HUMAN
reactivity :
Human, Mouse, Rat, Cow, Dog
sequence :
PRAYSDPDPAKDPR
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB)
app notes :
Western Blot: Approx 45kDa band observed in Human, Mouse and Rat Heart lysates (calculated MW of 34.9kDa according to NP_004378.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Shiojima et al, Circ Res. 1996 Nov;79(5):920-9.; PMID: 8888684). Recommended concentration: 0.3-1ug/ml. Peptide ELISA: antibody detection limit dilution 1:32000.
other info1 :
Epitope: Internal Region. Immunogen: Peptide with sequence C-PRAYSDPDPAKDPR, from the internal region of the protein sequence according to NP_004378.1; NP_001159647.1; NP_001159648.1. Corresponding Immunizing Peptide: Catalog #MBS426485
other info2 :
Important Note: This antibody is expected to recognize all three reported isoforms (NP_004378.1; NP_001159647.1; NP_001159648.1).
ncbi acc num :
NP_004378.1
ncbi gb acc num :
NM_004387.3
ncbi mol weight :
11,681 Da
ncbi pathways :
Cardiac Progenitor Differentiation Pathway 712094!!Heart Development Pathway 198802!!Regulation Of Nuclear SMAD2/3 Signaling Pathway 137963!!SRF And MiRs In Smooth Muscle Differentiation And Proliferation Pathway 672464
ncbi summary :
This gene encodes a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]