antibody

Goat anti-Clusterin/ApoJ (mouse) Antibody

MBS421997

0.1 mg

300 USD

polyclonal

goat

nonconjugated

mouse, rat

western blot, enzyme immunoassay

Antibody

Goat anti-Clusterin/ApoJ (mouse) Antibody

Clusterin/ApoJ

ApoJ; clusterin; Clu; AI893575; Cli; D14Ucla3; SP-40; Sgp-2; Sgp2; Sugp-2; Apolipoprotein J; complement lysis inhibitor; testosterone repressed prostate message; ApoJ antibody; clusterin antibody; Clu antibody; AI893575 antibody; Cli antibody; D14Ucla3 antibody; SP-40 antibody; Sgp-2 antibody; Sgp2 antibody; Sugp-2 antibody; Apolipoprotein J antibody; complement lysis inhibitor antibody; testosterone repressed prostate message antibody; Clusterin; Clusterin / ApoJ (mouse)

clusterin; Clusterin; clusterin; clusterin; Apolipoprotein J; Apo-J

Clu

APOJ

Clu; Clu; Cli; ApoJ; Sgp2; SP-40; Sgp-2; Sugp-2; AI893575; D14Ucla3; Apoj; Msgp-2; Apo-J; SGP-2

CLUS_MOUSE

Polyclonal

Goat

Tested: Rat; Expected from sequence similarity: Mouse, Rat

448

EKALQEYRRKSRAE

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB)

Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx.37kDa band observed in Mouse Brain and Testis lysates and in Rat Brain and Ovary lysates (calculated MW of 51.6kDa according to Mouse NP_038520.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miyata et al, Circulation.2001 Sep 18;104(12): 1407-12.; PMID: 11560857). Recommended concentration: 0.3-1ug/ml.

Immunogen: Peptide with sequence C-EKALQEYRRKSRAE, from the C Terminus of the protein sequence according to NP_038520.1. Epitope: C Terminus

7304967

NP_038520.1

NM_013492.1

51,656 Da

Complement And Coagulation Cascades Pathway (83270); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (1367536); Hemostasis Pathway (1368135); Immune System Pathway (1367472); Innate Immune System Pathway (1367502); Platelet Activation, Signaling And Aggregation Pathway (1368145); Platelet Degranulation Pathway (1368157); Response To Elevated Platelet Cytosolic Ca2+ Pathway (1368155); Terminal Pathway Of Complement (1367542)

The protein encoded by this gene is a secreted chaperone that can, under some stress conditions, also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. The encoded preproprotein undergoes proteolytic processing to generate a disulfide-linked heterodimeric mature protein comprised of alpha and beta subunits. Mice lacking the encoded protein exhibit increased severity of autoimmune myocarditis, faster progression of the acute inflammation to myocardial scarring and decreased brain injury following neonatal hypoxic-ischemic injury. [provided by RefSeq, Nov 2015]

CLU: Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress- induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. Belongs to the clusterin family. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Mitochondrial; Secreted; Secreted, signal peptide; Apoptosis. Cellular Component: cell surface; cytoplasm; cytoplasmic vesicle; endoplasmic reticulum; extracellular matrix; extracellular region; extracellular space; growth cone; intracellular; intracellular membrane-bound organelle; membrane; mitochondrion; neuron projection; nucleus; perinuclear region of cytoplasm; protein complex. Molecular Function: misfolded protein binding; protein binding; protein N-terminus binding; ubiquitin protein ligase binding. Biological Process: activation of NF-kappaB transcription factor; cell death; cell morphogenesis; chaperone-mediated protein complex assembly; endocrine pancreas development; microglial cell activation; myelin maintenance in the central nervous system; negative regulation of apoptosis; negative regulation of protein homooligomerization; neurite morphogenesis; positive regulation of apoptosis; positive regulation of cell differentiation; positive regulation of cell proliferation; positive regulation of nitric oxide biosynthetic process; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of tumor necrosis factor production; protein import; protein stabilization; response to misfolded protein

0.1 mg

300 USD