catalog number :
MBS421968
products full name :
Goat anti-EBF1/COE1 Antibody
products short name :
EBF1/COE1
products name syn :
EBF1; early B-cell factor 1; COE1; EBF; O/E-1; OLF1; Collier, Olf and EBF transcription factor 1; early B-cell factor olfactory neuronal transcription factor 1; EBF1 antibody; early B-cell factor 1 antibody; COE1 antibody; EBF antibody; O/E-1 antibody; OLF1 antibody; Collier; Olf and EBF transcription factor 1 antibody; early B-cell factor olfactory neuronal transcription factor 1 antibody; EBF1 / COE1
other names :
transcription factor COE1 isoform 2; Transcription factor COE1; transcription factor COE1; early B-cell factor 1; Early B-cell factor
products gene name :
EBF1
products gene name syn :
COE1
other gene names :
EBF1; EBF1; EBF; COE1; OLF1; O/E-1; COE1; EBF; O/E-1; OE-1
uniprot entry name :
COE1_HUMAN
reactivity :
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
app notes :
Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx.75kDa band observed in Human Adipose lysates (calculated MW of 64.5kDa according to NP_076870.1). Recommended concentration: 0.3-0.1ug/ml. Immunoprecipitation: Anonymous customer used this product successfully in IP from induced 3T3-L1 expressing FLAG-EBF1.
other info1 :
Immunogen: Peptide with sequence C-HNQLPALANTSV, from the internal region of the protein sequence according to NP_076870.1. Epitope: Internal region
other info2 :
Note: This antibody is expected not to cross-react with EBF2, EBF3 and EBF4.
ncbi acc num :
NP_076870.1
ncbi gb acc num :
NM_024007.4
ncbi mol weight :
61,059 Da
ncbi pathways :
Adipogenesis Pathway (198832); Developmental Biology Pathway (1270302); Transcriptional Regulation Of White Adipocyte Differentiation Pathway (1270345)
uniprot summary :
EBF1: Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Belongs to the COE family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Transcription regulation. Chromosomal Location of Human Ortholog: 5q34. Cellular Component: nucleus. Molecular Function: DNA binding; metal ion binding; protein binding. Biological Process: multicellular organismal development; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter