catalog number :
MBS421948
products full name :
Goat anti-Ramp1 (C-Term., mouse) Antibody
products short name :
Ramp1
products name syn :
Ramp1; receptor (calcitonin) activity modifying protein 1; 9130218E19Rik, MGC38240; receptor-activity modifying protein 1; Ramp1 antibody; receptor (calcitonin) activity modifying protein 1 antibody; 9130218E19Rik; MGC38240 antibody; receptor-activity modifying protein 1 antibody; Ramp1 (C-Term., mouse)
other names :
receptor activity-modifying protein 1 isoform 1; Receptor activity-modifying protein 1; receptor activity-modifying protein 1; receptor (calcitonin) activity modifying protein 1
products gene name :
Ramp1
other gene names :
Ramp1; Ramp1; 9130218E19Rik
uniprot entry name :
RAMP1_MOUSE
reactivity :
Tested: Rat; Expected from sequence similarity: Human, Mouse, Rat
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB)
app notes :
Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Experiments gave bands at approx.18kDa and 40kDa in Rat Brain lysate after 0.3ug/ml antibody staining. The appearance of a band with much higher molecular weight corresponds to earlier findings in literature with different antibodies (Hilairet, J Biol Chem.2001 Nov 9;276(45): 42182-90.; PMID: 11535606). This protein has a calculated MW of 16.9kDa according to Mouse NP_058590.1 and 16.8kDa according to Rat NP_113833.1. Recommended concentration: 0.3-1ug/ml.
other info1 :
Immunogen: Peptide with sequence C-DYQSKRTEGIV, from the C Terminus of the protein sequence according to NP_058590.1; NP_848488.2. Epitope: C Terminus
other info2 :
Note: This antibody is expected to recognize isoform 1 (NP_058590.1) only.
ncbi acc num :
NP_058590.1
ncbi gb acc num :
NM_016894.3
ncbi mol weight :
16,892 Da
ncbi pathways :
Calcitonin-like Ligand Receptors Pathway (1367081); Class B/2 (Secretin Family Receptors) Pathway (1367080); G Alpha (s) Signalling Events Pathway (1367085); GPCR Downstream Signaling Pathway (1367084); GPCR Ligand Binding Pathway (1367054); Myometrial Relaxation And Contraction Pathways (198333); Signal Transduction Pathway (1366876); Signaling By GPCR Pathway (1367053); Vascular Smooth Muscle Contraction Pathway (96531); Vascular Smooth Muscle Contraction Pathway (96866)
uniprot summary :
RAMP1: Transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. Acts as a receptor for calcitonin-gene-related peptide (CGRP) together with CALCRL. Belongs to the RAMP family. Protein type: Receptor, GPCR; Membrane protein, integral. Cellular Component: cell surface; extracellular space; integral to membrane; membrane; plasma membrane; receptor complex. Molecular Function: calcitonin receptor activity; calcitonin receptor binding; coreceptor activity; protein transporter activity; receptor activity. Biological Process: angiogenesis; calcium ion transport; G-protein coupled receptor protein signaling pathway; intracellular protein transport; positive regulation of cAMP biosynthetic process; positive regulation of protein amino acid glycosylation; protein transport; receptor internalization; regulation of G-protein coupled receptor protein signaling pathway; transport