antibody

Goat anti-FBXO32 Antibody

MBS421930

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, mouse, rat, cow

western blot, enzyme immunoassay

Antibody

Goat anti-FBXO32 Antibody

FBXO32

FBXO32; F-box protein 32; FLJ32424; Fbx32; MAFbx; MGC33610; F-box only protein 32; atrogin 1; muscle atrophy F-box protein; FBXO32 antibody; F-box protein 32 antibody; FLJ32424 antibody; Fbx32 antibody; MAFbx antibody; MGC33610 antibody; F-box only protein 32 antibody; atrogin 1 antibody; muscle atrophy F-box protein antibody

F-box only protein 32 isoform 1; F-box only protein 32; F-box only protein 32; F-box protein 32; Atrogin-1; Muscle atrophy F-box protein; MAFbx

FBXO32

FBXO32; FBXO32; Fbx32; MAFbx; MAFbx

FBX32_HUMAN

Polyclonal

Goat

Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Cow, Pig

355

NSKTKTQYFHQEK

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB)

Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: approx. 40kDa band observed in Human and Mouse Skeletal Muscle lysates (calculated MW of 41.6kDa according to Human NP_478136.1 and of 41.5kDa according to Mouse NP_080622.1). Recommended concentration: 1-3ug/ml.

Immunogen: Peptide with sequence C-NSKTKTQYFHQEK, from the internal region of the protein sequence according to NP_478136.1. Epitope: Internal region

Note: This antibody is expected to recognize reported isoform 1 (NP_478136.1).

17158037

NP_478136.1

NM_058229.3

30,804 Da

FoxO Family Signaling Pathway (138036); FoxO Signaling Pathway (921162); Monoamine Transport Pathway (198851)

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

FBXO32: Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Ubiquitin conjugating system. Chromosomal Location of Human Ortholog: 8q24.13. Cellular Component: cytoplasm; nucleoplasm; nucleus; SCF ubiquitin ligase complex; Z disc. Molecular Function: protein binding; ubiquitin-protein ligase activity. Biological Process: protein ubiquitination; response to denervation involved in regulation of muscle adaptation; ubiquitin-dependent protein catabolic process

0.1 mg

300 USD