Goat anti-ERN1/IRE1a Antibody | MyBioSource MBS421918 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-ERN1/IRE1a Antibody

catalog :

MBS421918

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, cow

application :

western blot, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS421918

products type :

Antibody

products full name :

Goat anti-ERN1/IRE1a Antibody

products short name :

ERN1/IRE1a

products name syn :

Goat Anti-IRE1 / ERN1 Antibody; ERN1; IRE1; endoplasmic reticulum to nucleus signaling 1; FLJ30999; IRE1P; MGC163277; MGC163279; ER to nucleus signalling 1; endoplasmic reticulum to nucleus signalling 1; inositol-requiring 1; protein kinase/endoribonuclease; IRE1a, IRE1-alpha; ERN1 antibody; IRE1 antibody; endoplasmic reticulum to nucleus signaling 1 antibody; FLJ30999 antibody; IRE1P antibody; MGC163277 antibody; MGC163279 antibody; ER to nucleus signalling 1 antibody; endoplasmic reticulum to nucleus signalling 1 antibody; inositol-requiring 1 antibody; protein kinase/endoribonuclease antibody; IRE1a; IRE1-alpha antibody; ERN1 / IRE1a

other names :

serine/threonine-protein kinase/endoribonuclease IRE1; Serine/threonine-protein kinase/endoribonuclease IRE1; serine/threonine-protein kinase/endoribonuclease IRE1; endoplasmic reticulum to nucleus signaling 1; Endoplasmic reticulum-to-nucleus signaling 1; Inositol-requiring protein 1; hIRE1p; Ire1-alpha; IRE1a

products gene name :

ERN1

products gene name syn :

IRE1a

other gene names :

ERN1; ERN1; IRE1; IRE1P; IRE1a; hIRE1p; hIRE1p; IRE1a

uniprot entry name :

ERN1_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

sequence length :

977

sequence :

PNAHGKIKAMISD

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx.110kDa band observed in Human Pancreas lysates (calculated MW of 110kDa according to NP_001424.3). Recommended concentration: 0. 03-0.1ug/ml.

other info1 :

Immunogen: Peptide with sequence C-PNAHGKIKAMISD, from the internal region of the protein sequence according to NP_001424.3. Epitope: Internal region

ncbi gi num :

153946421

ncbi acc num :

NP_001424.3

ncbi gb acc num :

NM_001433.3

ncbi mol weight :

6,649 Da

ncbi pathways :

Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Apoptosis Pathway (83060); Apoptosis Pathway (470); Corticotropin-releasing Hormone Pathway (920957); IRE1alpha Activates Chaperones Pathway (1268759); Metabolism Of Proteins Pathway (1268677); Non-alcoholic Fatty Liver Disease (NAFLD) Pathway (862188); Non-alcoholic Fatty Liver Disease (NAFLD) Pathway (862314)

ncbi summary :

The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq, Jul 2008]

uniprot summary :

IRE1: a ser/thr protein kinase that possess endonuclease activity. Important in altering gene expression as a response to endoplasmic reticulum based stress signals. Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain, which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. The kinase domain is activated by trans-autophosphorylation. Kinase activity is required for activation of the endoribonuclease domain. Ubiquitously expressed. High levels observed in pancreatic tissue. Functionally connected with insulin biosynthesis in pancreatic beta cells. Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Also binds HSPA5, a negative regulator of the unfolded protein response. This interaction may disrupt homodimerization and prevent activation of IRE1. Protein type: Chaperone; Apoptosis; Membrane protein, integral; EC 2.7.11.1; Protein kinase, Other; Ribonuclease; Kinase, protein; Endoplasmic reticulum; Protein kinase, Ser/Thr (non-receptor); Other group; IRE family. Chromosomal Location of Human Ortholog: 17q24.2. Cellular Component: cytoplasm; endoplasmic reticulum; endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane; mitochondrion; nuclear inner membrane. Molecular Function: ADP binding; ATP binding; endoribonuclease activity; enzyme binding; Hsp70 protein binding; Hsp90 protein binding; identical protein binding; magnesium ion binding; protein binding; protein homodimerization activity; protein serine/threonine kinase activity. Biological Process: activation of JNK activity; cell cycle arrest; endothelial cell proliferation; mRNA catabolic process; mRNA cleavage; positive regulation of RNA splicing; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of macroautophagy; regulation of transcription, DNA-dependent; transcription, DNA-dependent; unfolded protein response, activation of signaling protein activity

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage