Goat anti-SLC10A2/ASBT Antibody | MyBioSource MBS421745 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-SLC10A2/ASBT Antibody

catalog :

MBS421745

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human

application :

immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS421745

products type :

Antibody

products full name :

Goat anti-SLC10A2/ASBT Antibody

products short name :

SLC10A2/ASBT

products name syn :

SLC10A2; ASBT; solute carrier family 10 (sodium/bile acid cotransporter family), member 2; ISBT; NTCP2; OTTHUMP00000040691; ileal apical sodium-dependent bile acid transporter; ileal sodium-dependent bile acid transporter; SLC10A2 antibody; ASBT antibody; solute carrier family 10 (sodium/bile acid cotransporter family); member 2 antibody; ISBT antibody; NTCP2 antibody; OTTHUMP00000040691 antibody; ileal apical sodium-dependent bile acid transporter antibody; ileal sodium-dependent bile acid transporter antibody; SLC10A2 / ASBT

other names :

ileal sodium/bile acid cotransporter; Ileal sodium/bile acid cotransporter; ileal sodium/bile acid cotransporter; solute carrier family 10 member 2; Apical sodium-dependent bile acid transporter; ASBT; Ileal Na(+)/bile acid cotransporter; Ileal sodium-dependent bile acid transporter; IBAT; ISBT; Na(+)-dependent ileal bile acid transporter; Sodium/taurocholate cotransporting polypeptide, ileal; Solute carrier family 10 member 2

products gene name :

SLC10A2

products gene name syn :

ASBT

other gene names :

SLC10A2; SLC10A2; ASBT; IBAT; ISBT; PBAM; NTCP2; ASBT; ISBT; NTCP2; ASBT; IBAT; ISBT

uniprot entry name :

NTCP2_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human; Expected from sequence similarity: Human

sequence length :

348

sequence :

YKANGGFQPDEK

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Immunohistochemistry (IHC)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 16000. Immunohistochemistry: In paraffin embedded Human Small Intestine shows staining of cells in the laminia propria between the intestinal vili. Recommended concentration, 3-6ug/ml. Western Blot: Preliminary experiments gave an approx. 60kDa band in Human Kidney lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 37.7kDa according to NP_000443.1. The 60kDa band was successfully blocked by incubation with the immunizing peptide.

other info1 :

Immunogen: Peptide with sequence C-YKANGGFQPDEK, from the C Terminus of the protein sequence according to NP_000443.1. Epitope: C Terminus

ncbi gi num :

4506973

ncbi acc num :

NP_000443.1

ncbi gb acc num :

NM_000452.2

ncbi mol weight :

37,714 Da

ncbi pathways :

Bile Acid And Bile Salt Metabolism Pathway (1270040); Bile Secretion Pathway (193146); Bile Secretion Pathway (193095); Metabolism Pathway (1269956); Metabolism Of Lipids And Lipoproteins Pathway (1270001); Recycling Of Bile Acids And Salts Pathway (1270045)

ncbi summary :

This gene encodes a sodium/bile acid cotransporter. This transporter is the primary mechanism for uptake of intestinal bile acids by apical cells in the distal ileum. Bile acids are the catabolic product of cholesterol metabolism, so this protein is also critical for cholesterol homeostasis. Mutations in this gene cause primary bile acid malabsorption (PBAM); muatations in this gene may also be associated with other diseases of the liver and intestines, such as familial hypertriglyceridemia (FHTG). [provided by RefSeq, Mar 2010]

uniprot summary :

ASBT: Plays a critical role in the sodium-dependent reabsorption of bile acids from the lumen of the small intestine. Plays a key role in cholesterol metabolism. Defects in SLC10A2 are a cause of primary bile acid malabsorption (PBAM). PBAM is an idiopathic intestinal disorder associated with congenital diarrhea, steatorrhea, interruption of the enterohepatic circulation of bile acids, and reduced plasma cholesterol levels. Belongs to the bile acid:sodium symporter (BASS) (TC 2.A.28) family. Protein type: Membrane protein, multi-pass; Transporter; Membrane protein, integral. Chromosomal Location of Human Ortholog: 13q33. Cellular Component: apical plasma membrane; integral to plasma membrane; microvillus; plasma membrane. Molecular Function: bile acid:sodium symporter activity. Biological Process: bile acid and bile salt transport; sodium ion transport; transmembrane transport; transport. Disease: Bile Acid Malabsorption, Primary

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage