catalog number :
MBS421725
products full name :
Goat anti-MARCH10 Antibody
products short name :
10-Mar
products name syn :
MARCH10; membrane-associated ring finger (C3HC4) 10; FLJ35757; MARCH-X; RNF190; ring finger protein 190; membrane-associated ring finger (C3HC4) 10; FLJ35757; MARCH-X; RNF190; ring finger protein 190
other names :
probable E3 ubiquitin-protein ligase MARCH10 isoform 1; Probable E3 ubiquitin-protein ligase MARCH10; probable E3 ubiquitin-protein ligase MARCH10; ring finger protein 190; membrane-associated RING-CH protein X; membrane-associated RING finger protein 10; membrane-associated ring finger (C3HC4) 10, E3 ubiquitin protein ligase; Membrane-associated RING finger protein 10; Membrane-associated RING-CH protein X; MARCH-X; RING finger protein 190
products gene name :
10-Mar
other gene names :
MARCH10; MARCH10; RNF190; MARCH-X; RNF190; MARCH-X
uniprot entry name :
MARHA_HUMAN
reactivity :
Human, Rat, Cow
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB)
app notes :
Western Blot: Approx. 90 kDa band observed in Rat Testis lysates (calculated MW of 90.5 kDa according to Human NP_689811.2 and of 87.9 kDa according to Mouse NP_766156.2). Recommended concentration: 0.3-1ug/ml. An additional band of unknown identity was also consistently observed at 37 kDa. This band was successfully blocked by incubation with the immunising peptide. Peptide ELISA: antibody detection limit dilution 1:8000.
other info1 :
Epitope: Internal Region. Immunogen: Peptide with sequence C-SRNYPQPRTEE, from the internal region of the protein sequence according to NP_001094345.1; NP_689811.2. Corresponding Immunizing Peptide: Catalog #MBS425084
other info2 :
Important Note: This antibody is expected to recognise both reported isoforms (NP_001094345.1; NP_689811.2), which are identical.
ncbi acc num :
NP_001094345.1
ncbi gb acc num :
NM_001100875.2
ncbi mol weight :
90,511 Da
ncbi summary :
MARCH10 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Morokuma et al., 2007 [PubMed 17604280]).[supplied by OMIM, Apr 2010]