catalog number :
MBS421669
products full name :
Goat anti-Proenkephalin (mouse) Antibody
products short name :
Proenkephalin
products name syn :
proenkephalin; proenkephalin antibody; Proenkephalin; Proenkephalin (mouse)
other names :
proenkephalin-A; Proenkephalin-A; proenkephalin-A; preproenkephalin; SynenkephalinMet-enkephalinAlternative name(s):Opioid growth factor; OGF
products gene name :
PENK
other gene names :
Penk; Penk; ENK; PPA; Penk1; AI326464; Penk1; OGF
uniprot entry name :
PENK_MOUSE
reactivity :
Tested: Mouse, Rat; Expected from sequence similarity: Mouse, Rat
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Immunohistochemistry (IHC)
app notes :
Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: Frozen section of Rat Brain (Striatum) shows staining of dense enkephalinergic axon plexus. Frozen section of the Mouse Brain (Stria terminalis) shows staining of a dense enkephalinergic fibre network in the bed nucleus. Recommended concentration, 0.02-0.05ug/ml. Western Blot: Preliminary experiments gave an approx 40kDa band in Rat Brain lysates after 0.2ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.8kDa according to Mouse NP_001002927.1 and 30.9kDa according to Rat NP_058835.1. The 40kDa band was successfully blocked by incubation with the immunizing peptide.
other info1 :
Immunogen: Peptide with sequence C-YKDSSKQDESH, from the internal region of the protein sequence according to NP_001002927.1. Epitope: Internal region
ncbi acc num :
NP_001002927.1
ncbi gb acc num :
NM_001002927.2
ncbi mol weight :
31,004 Da
ncbi pathways :
Class A/1 (Rhodopsin-like Receptors) Pathway (1367055); G Alpha (i) Signalling Events Pathway (1367086); GPCR Downstream Signaling Pathway (1367084); GPCR Ligand Binding Pathway (1367054); Peptide Ligand-binding Receptors Pathway (1367056); Signal Transduction Pathway (1366876); Signaling By GPCR Pathway (1367053)
uniprot summary :
PENK: Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum. Belongs to the opioid neuropeptide precursor family. Protein type: Secreted; Secreted, signal peptide; Hormone