Goat anti-ACO1/Aconitase 1 Antibody | MyBioSource MBS421648 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-ACO1/Aconitase 1 Antibody

catalog :

MBS421648

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, cow

application :

western blot, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS421648

products type :

Antibody

products full name :

Goat anti-ACO1/Aconitase 1 Antibody

products short name :

ACO1/Aconitase 1

products name syn :

ACO1; aconitase 1, soluble; ACONS; IREB1; IREBP; IREBP1; IRP1; OTTHUMP00000021176; OTTHUMP00000021177; OTTHUMP00000045233; aconitase 1; aconitate hydratase; citrate hydro-lyase; ferritin repressor protein; iron regulatory protein 1; iron-responsive elemen; ACO1 antibody; aconitase 1; soluble antibody; ACONS antibody; IREB1 antibody; IREBP antibody; IREBP1 antibody; IRP1 antibody; OTTHUMP00000021176 antibody; OTTHUMP00000021177 antibody; OTTHUMP00000045233 antibody; aconitase 1 antibody; aconitate hydratase antibody; citrate hydro-lyase antibody; ferritin repressor protein antibody; iron regulatory protein 1 antibody; iron-responsive element binding protein 1 antibody; Aconitase 1; ACO1 / Aconitase 1

other names :

cytoplasmic aconitate hydratase; Cytoplasmic aconitate hydratase; cytoplasmic aconitate hydratase; aconitase 1; Citrate hydro-lyase; Ferritin repressor protein; Iron regulatory protein 1; IRP1; Iron-responsive element-binding protein 1; IRE-BP 1

products gene name :

ACO1

other gene names :

ACO1; ACO1; IRP1; ACONS; HEL60; IREB1; IREBP; IREBP1; IREB1; Aconitase; IRP1; IRE-BP 1

uniprot entry name :

ACOC_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Cow

sequence length :

889

sequence :

QVDFNRRADSLQKNQ

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx 100kDa band observed in Human Liver lysates (calculated MW of 98.4kDa according to NP_002188.1). Recommended concentration: 0.1-0.3ug/ml.

other info1 :

Immunogen: Peptide with sequence C-QVDFNRRADSLQKNQ, from the internal region of the protein sequence according to NP_002188.1. Epitope: Internal region

ncbi gi num :

8659555

ncbi acc num :

NP_002188.1

ncbi gb acc num :

NM_002197.2

ncbi mol weight :

98,399 Da

ncbi pathways :

2-Oxocarboxylic Acid Metabolism Pathway (714485); 2-Oxocarboxylic Acid Metabolism Pathway (717400); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Citrate Cycle (TCA Cycle) Pathway (82927); Citrate Cycle (TCA Cycle) Pathway (288); Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway (855811); Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway (468202)

ncbi summary :

The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014]

uniprot summary :

IREB1: Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding. Belongs to the aconitase/IPM isomerase family. Protein type: RNA-binding; Endoplasmic reticulum; Lyase; Carbohydrate Metabolism - citrate (TCA) cycle; EC 4.2.1.3; Carbohydrate Metabolism - glyoxylate and dicarboxylate; Translation. Chromosomal Location of Human Ortholog: 9p21.1. Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; Golgi apparatus; mitochondrion. Molecular Function: 4 iron, 4 sulfur cluster binding; aconitate hydratase activity; iron-responsive element binding; metal ion binding; protein binding; RNA binding. Biological Process: cellular iron ion homeostasis; citrate metabolic process; intestinal absorption; post-embryonic development; regulation of translation; response to iron(II) ion; tricarboxylic acid cycle

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage