catalog number :
MBS421634
products full name :
Goat anti-JARID2 Antibody
products short name :
JARID2
products name syn :
JARID2; jumonji; AT rich interactive domain 2; JMJ; OTTHUMP00000016058; jumonji homolog; jumonji; AT rich interactive domain 2 protein; jumonji-like protein; JARID2; jumonji, AT rich interactive domain 2; JMJ; OTTHUMP00000016058; jumonji homolog; jumonji, AT rich interactive domain 2 protein; jumonji-like protein
other names :
protein Jumonji isoform 1; Protein Jumonji; protein Jumonji; jumonji homolog; jumonji-like protein; jumonji/ARID domain-containing protein 2; jumonji, AT rich interactive domain 2; Jumonji/ARID domain-containing protein 2
products gene name :
JARID2
other gene names :
JARID2; JARID2; JMJ; JMJ
uniprot entry name :
JARD2_HUMAN
reactivity :
Human, Mouse, Rat, Dog, Pig, Cow
sequence :
HRSAQDLRKQVSKVN
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA)
app notes :
Western Blot: Preliminary experiments gave an approx 29kDa band in Mouse and Rat Spinal Cord and in Mouse Fetal Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 137kDa according to Mouse NP_068678.1. The 29kDa band was successfully blocked by incubation with the immunizing peptide. Peptide ELISA: antibody detection limit dilution 1:32000.
other info1 :
Epitope: Internal Region. Immunogen: Peptide with sequence C-HRSAQDLRKQVSKVN, from the internal region of the protein sequence according to NP_004964.2.
other info2 :
Corresponding Immunizing Peptide: Catalog #MBS425426
ncbi acc num :
NP_004964.2
ncbi gb acc num :
NM_004973.3
ncbi mol weight :
119,689 Da
ncbi pathways :
Epigenetic Regulation Of Gene Expression Pathway 980449!!Gene Expression Pathway 105937!!PRC2 Methylates Histones And DNA Pathway 980450!!Signaling Pathways Regulating Pluripotency Of Stem Cells 1026136!!Signaling Pathways Regulating Pluripotency Of Stem Cells 1033502
ncbi summary :
This gene encodes a Jumonji- and AT-rich interaction domain (ARID)-domain-containing protein. The encoded protein is a DNA-binding protein that functions as a transcriptional repressor. This protein interacts with the Polycomb repressive complex 2 (PRC2) which plays an essential role in regulating gene expression during embryonic development. This protein facilitates the recruitment of the PRC2 complex to target genes. Alternate splicing results in multiple transcript variants. Mutations in this gene are associated with chronic myeloid malignancies. [provided by RefSeq, May 2012]