catalog number :
MBS421633
products full name :
Goat anti-ACAT1 (aa253-266) Antibody
products short name :
ACAT1
products name syn :
ACAT1; acetyl-Coenzyme A acetyltransferase 1; ACAT; MAT; T2; THIL; acetoacetyl Coenzyme A thiolase; Goat Anti-ACAT1 (aa257-269); acetyl-Coenzyme A acetyltransferase 1; ACAT; MAT; T2; THIL; acetoacetyl Coenzyme A thiolase
other names :
acetyl-CoA acetyltransferase, mitochondrial; Acetyl-CoA acetyltransferase, mitochondrial; acetyl-CoA acetyltransferase, mitochondrial; acetoacetyl-CoA thiolase; acetoacetyl Coenzyme A thiolase; acetyl-Coenzyme A acetyltransferase 1; mitochondrial acetoacetyl-CoA thiolase; acetyl-CoA acetyltransferase 1; Acetoacetyl-CoA thiolase; T2
products gene name :
ACAT1
other gene names :
ACAT1; ACAT1; T2; MAT; ACAT; THIL; ACAT; MAT
uniprot entry name :
THIL_HUMAN
reactivity :
Human, Mouse, Rat, Dog, Cow
sequence :
DEEYKRVDFSKVPK
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
app notes :
Western Blot: Approx 45kDa band observed in Human, Mouse and Rat Liver lysates (calculated MW of 45.2kDa according to Human NP_000010.1 and 44.81kDa to Mouse NP_659033.1). Recommended concentration: 0.1-0.3ug/ml. Peptide ELISA: antibody detection limit dilution 1:2000. Immunofluorescence: Customer finds particulate cytoplasm staining in HeLa.
other info1 :
Epitope: Internal Region. Immunogen: Peptide with sequence C-DEEYKRVDFSKVPK, from the internal region of the protein sequence according to NP_000010.1.
other info2 :
Corresponding Immunizing Peptide: Catalog #MBS425066
ncbi acc num :
NP_000010.1
ncbi gb acc num :
NM_000019.3
ncbi mol weight :
17,175 Da
ncbi pathways :
Branched-chain Amino Acid Catabolism Pathway 106179!!Butanoate Metabolism Pathway 83007!!Butanoate Metabolism Pathway 391!!C5 Isoprenoid Biosynthesis, Mevalonate Pathway 413387!!C5 Isoprenoid Biosynthesis, Mevalonate Pathway 468288!!Carbon Metabolism Pathway 814926!!Carbon Metabolism Pathway 817567!!Fatty Acid Beta Oxidation Pathway 198865!!Fatty Acid Degradation Pathway 82935!!Fatty Acid Degradation Pathway 296
ncbi summary :
This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. [provided by RefSeq, Feb 2009]