antibody

Goat anti-Ankyrin 1 / ANK1 Antibody

MBS421627

0.1 mg

225 USD

polyclonal

goat

nonconjugated

human, mouse, rat, cow

ELISA, enzyme immunoassay

Antibody

Goat anti-Ankyrin 1 / ANK1 Antibody

Ankyrin 1/ANK1

ANK1; ankyrin 1; erythrocytic; ANK; SPH1; SPH2; ankyrin 1; ankyrin-1; erythrocytic; ankyrin-R; ankyrin 1, erythrocytic; ANK; SPH1; SPH2; ankyrin 1; ankyrin-1, erythrocytic; ankyrin-R

ankyrin-1 isoform 3; Ankyrin-1; ankyrin-1; ANK-1; ankyrin-R; erythrocyte ankyrin; ankyrin 1, erythrocytic; Ankyrin-R; Erythrocyte ankyrin

ANK1

ANK1; ANK1; ANK; SPH1; SPH2; ANK; ANK-1

ANK1_HUMAN

Polyclonal

Goat

Human, Mouse, Rat, Cow

1880

QIVKRASLKRGKQ

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA)

Western Blot: Preliminary experiments gave approx. 19-23 kDa bands in Human Skeletal Muscle lysates after 0.01ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for these bands we observe given the observed 28+30 kDa bands in the literature for this tissue (PMID: 9430667) and given the calculated size of 17.6 kDa according to NP_065211.2. The 19-22 kDa bands were successfully blocked by incubation with the immunizing peptide. Peptide ELISA: antibody detection limit dilution 1:128000.

Epitope: C Terminus. Immunogen: Peptide with sequence C-QIVKRASLKRGKQ, from the C Terminus of the protein sequence according to NP_000028.3; NP_065208.2; NP_065209.2; NP_065210.2; NP_065211.2; NP_065213.2. Corresponding Immunizing Peptide: Catalog #MBS426113

Important Note: This antibody is expected to recognise isoform 3 (NP_000028.3) and isoform 5 (NP_065211.2).

70780357

NP_000028.3

NM_000037.3

17,854 Da

Axon Guidance Pathway 105688!!CHL1 Interactions Pathway 161007!!Developmental Biology Pathway 477129!!Interaction Between L1 And Ankyrins Pathway 161005!!L1CAM Interactions Pathway 161003!!Neurofascin Interactions Pathway 161008!!NrCAM Interactions Pathway 161009!!Proteoglycans In Cancer Pathway 782000!!Proteoglycans In Cancer Pathway 782054

Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified. [provided by RefSeq, Dec 2008]

0.1 mg

225 USD