catalog number :
MBS421620
products full name :
Goat anti-SPHK1 Antibody
products short name :
SPHK1
products name syn :
SPHK1; sphingosine kinase 1; SPHK; SK1; SKase; Goat Anti-SPHK1 (C-term.); SPHK1; sphingosine kinase 1; SPHK; SK1; SKase
other names :
sphingosine kinase 1 isoform 1; Sphingosine kinase 1; sphingosine kinase 1; SK 1; SPK 1; sphingosine kinase 1
products gene name :
SPHK1
other gene names :
SPHK1; SPHK1; SPHK; SPHK; SPK; SK 1; SPK 1
uniprot entry name :
SPHK1_HUMAN
reactivity :
Human, Mouse, Rat, Dog, Cow
sequence :
DVDLESEKYRRLGE
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
app notes :
Western Blot: Approx. 40 kDa band observed in Human Peripheral Blood Mononucleocyte lysates (calculated MW of 43.9 kDa according to NP_068807.2). Recommended concentration: 2-3ug/ml. Peptide ELISA: antibody detection limit dilution 1:16000. Immunoprecipitation: Has been used in nuclear lysates of HeLa cells and a band of ~45kDa band has been detected.
other info1 :
Epitope: Internal Region. Immunogen: Peptide with sequence C-DVDLESEKYRRLGE, from the internal region of the protein sequence according to NP_068807.2; NP_892010.2; NP_001136073.1. Corresponding Immunizing Peptide: Catalog #MBS426444
other info2 :
Important Note: This antibody is expected to recognise all three reported isoforms (NP_068807.2; NP_892010.2; NP_001136073.1). Reported variants represent identical protein (NP_001136073.1; NP_001136074.1).
ncbi acc num :
NP_068807.2
ncbi gb acc num :
NM_021972.3
ncbi mol weight :
43,944 Da
ncbi pathways :
Association Of TriC/CCT With Target Proteins During Biosynthesis Pathway 106247!!Calcium Signaling Pathway 83050!!Calcium Signaling Pathway 459!!Chaperonin-mediated Protein Folding Pathway 106242!!Fc Gamma R-mediated Phagocytosis Pathway 114228!!Fc Gamma R-mediated Phagocytosis Pathway 106590!!Fc-epsilon Receptor I Signaling In Mast Cells Pathway 138050!!Metabolic Pathways 132956!!Metabolism Pathway 477135!!Metabolism Of Lipids And Lipoproteins Pathway 160976
ncbi summary :
The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]