antibody

Goat anti-PNPLA3/Adiponutrin Antibody

MBS421616

0.1 mg

300 USD

polyclonal

goat

nonconjugated

mouse, rat, dog

western blot, immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-PNPLA3/Adiponutrin Antibody

PNPLA3/Adiponutrin

Pnpla3; patatin-like phospholipase domain containing 3; Adpn; adiponutrin; Pnpla3 antibody; patatin-like phospholipase domain containing 3 antibody; Adpn antibody; adiponutrin antibody; Adiponutrin; PNPLA3 / Adiponutrin

patatin-like phospholipase domain-containing protein 3; Patatin-like phospholipase domain-containing protein 3; patatin-like phospholipase domain-containing protein 3; patatin-like phospholipase domain containing 3; Acylglycerol O-acyltransferase (EC:2.3.1.-); Adiponutrin; Calcium-independent phospholipase A2-epsilon; iPLA2-epsilon

PNPLA3

Pnpla3; Pnpla3; Adpn; Adpn; iPLA2-epsilon

PLPL3_MOUSE

Polyclonal

Goat

Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

384

EHDICPKVKSTN

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Peptide ELISA: Antibody detection limit dilution 1: 128000. Immunohistochemistry: In paraffin embedded Human Skin shows membranous staining of basak kayer cells. Recommended concentration, 4-6ug/ml. Western Blot: Approx 45kDa band observed in Mouse Heart lysates and in Mouse and Rat Adrenal gland lysates (calculated MW of 42.6kDa according to Mouse NP_473429.2 and 47.1kDa according to Rat NP_)001269253.1. Recommended concentration: 0.01-0.03ug/ml.

Immunogen: Peptide with sequence EHDICPKVKSTN, from the internal region of the protein sequence according to NP_473429.2. Epitope: Internal region

Note: This antibody is raised against a peptide derived from the Mouse protein sequence and this peptide differs from Human only in position 2.

144226245

NP_473429.2

NM_054088.3

45,772 Da

Acyl Chain Remodeling Of DAG And TAG Pathway (1367713); Acylglycerol Degradation Pathway (421788); Acylglycerol Degradation Pathway (468272); Adipogenesis Pathway (198299); Glycerolipid Metabolism Pathway (83185); Glycerolipid Metabolism Pathway (361); Glycerolipids/Glycerophospholipids Metabolism Pathway (522981); Glycerophospholipid Biosynthesis Pathway (1367711); Metabolic Pathways (132962); Metabolism Pathway (1367614)

PNPLA3: Multifunctional enzyme which has both triacylglycerol lipase and acylglycerol O-acyltransferase activities. Genetic variations in PNPLA3 are a cause of susceptibility to non-alcoholic fatty liver disease type 1 (NAFLD1). A condition characterized by an accumulation of excess triglyceride in the liver, a condition known as hepatic steatosis (or fatty liver), which is associated with adverse metabolic consequences, including insulin resistance and dyslipidemia. In a subset of individuals, hepatic steatosis promotes an inflammatory response in the liver, referred to as steatohepatitis, which can progress to cirrhosis and liver cancer. NAFLD is the most common form of liver disease in Western countries. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Lipid Metabolism - glycerolipid; Amino Acid Metabolism - tyrosine; Transferase; Membrane protein, integral; Amino Acid Metabolism - phenylalanine; EC 3.1.1.3; Lipid Metabolism - glycerophospholipid; Secondary Metabolites Metabolism - limonene and pinene degradation; Phospholipase. Cellular Component: integral to membrane; membrane. Molecular Function: 3-hydroxybutyryl-CoA thiolase activity; 3-ketopimelyl-CoA thiolase activity; acetyltransferase activity; acyl-CoA N-acyltransferase activity; acylglycerol O-acyltransferase activity; azetidine-2-carboxylic acid acetyltransferase activity; benzoyl acetate-CoA thiolase activity; C-acyltransferase activity; C-palmitoyltransferase activity; carnitine O-acyltransferase activity; dihydrolipoamide branched chain acyltransferase activity; dihydrolipoamide S-acyltransferase activity; diolein transacylation activity; glucosaminyl-phosphotidylinositol O-acyltransferase activity; hydrolase activity; keto acid formate lyase activity; L-2-aminoadipate N-acetyltransferase activity; malonyltransferase activity; mono-olein transacylation activity; myristoyltransferase activity; N-acetyltransferase activity; N-acyltransferase activity; N-palmitoyltransferase activity; N-succinyltransferase activity; O-acetyltransferase activity; O-acyltransferase activity; O-octanoyltransferase activity; O-palmitoyltransferase activity; O-sinapoyltransferase activity; O-succinyltransferase activity; octanoyltransferase activity; palmitoleoyl [acyl-carrier-protein]-dependent acyltransferase activity; palmitoyltransferase activity; peptidyl-lysine N6-myristoyltransferase activity; peptidyl-lysine N6-palmitoyltransferase activity; phospholipase A2 activity; protein-cysteine S-acyltransferase activity; protein-cysteine S-myristoyltransferase activity; Ras palmitoyltransferase activity; S-acetyltransferase activity; S-acyltransferase activity; S-malonyltransferase activity; S-succinyltransferase activity; serine O-acyltransferase activity; sinapoyltransferase activity; sterol O-acyltransferase activity; succinyltransferase activity; transferase activity; transferase activity, transferring acyl groups; triacylglycerol lipase activity. Biological Process: lipid catabolic process; lipid metabolic process; metabolic process; triacylglycerol biosynthetic process; triacylglycerol catabolic process

0.1 mg

300 USD