catalog number :
MBS421605
products full name :
Goat anti-PAM / PAL Antibody
products short name :
PAM/PAL
products name syn :
PAM; PAL; peptidylglycine alpha-amidating monooxygenase; PHM; pancreatic peptidylglycine alpha-amidating monooxygenase; peptidyl alpha-amidating enzyme; peptidyl-alpha-hydroxyglycine alpha-amidating lyase; peptidylglycine 2-hydroxylase; peptidylglycine alpha-hydroxylating monooxygenase; PAM; PAL; peptidylglycine alpha-amidating monooxygenase; PHM; pancreatic peptidylglycine alpha-amidating monooxygenase; peptidyl alpha-amidating enzyme; peptidyl-alpha-hydroxyglycine alpha-amidating lyase; peptidylglycine 2-hydroxylase; peptidylglycine alpha-hydroxylating monooxygenase
other names :
peptidyl-glycine alpha-amidating monooxygenase isoform a preproprotein; Peptidyl-glycine alpha-amidating monooxygenase; peptidyl-glycine alpha-amidating monooxygenase; peptidylamidoglycolate lyase; peptidylglycine 2-hydroxylase; peptidyl alpha-amidating enzyme; peptidylglycine alpha-hydroxylating monooxygenase; peptidyl-alpha-hydroxyglycine alpha-amidating lyase; pancreatic peptidylglycine alpha-amidating monooxygenase; peptidylglycine alpha-amidating monooxygenase; Peptidylamidoglycolate lyase; PAL
other gene names :
PAM; PAM; PAL; PHM; PAM; PHM; PAL
uniprot entry name :
AMD_HUMAN
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB)
app notes :
Western Blot: Approx 100kDa band observed in Human Kidney lysates (calculated MW of 101kDa according to preproprotein NP_620121.1). Recommended concentration: 0.3-1ug/ml. Peptide ELISA: antibody detection limit dilution 1:4000.
other info1 :
Epitope: C Terminus. Immunogen: Peptide with sequence C-DQEKEDDGSESEE, from the C Terminus of the protein sequence according to NP_000910.2; NP_620121.1; NP_620176.1; NP_620177.1. Corresponding Immunizing Peptide: Catalog #MBS425351
other info2 :
Important Note: This antibody is expected to recognise all reported isoforms (NP_000910.2; NP_620121.1; NP_620176.1 and NP_620177.1).
ncbi acc num :
NP_000910.2
ncbi gb acc num :
NM_000919.3
ncbi mol weight :
106,276 Da
ncbi summary :
This gene encodes a multifunctional protein. It has two enzymatically active domains with catalytic activities - peptidylglycine alpha-hydroxylating monooxygenase (PHM) and peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL). These catalytic domains work sequentially to catalyze neuroendocrine peptides to active alpha-amidated products. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene but some of their full length sequences are not yet known. [provided by RefSeq, Jul 2008]