antibody

Goat anti-DNMT1 Antibody

MBS421587

0.1 mg

300 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog, cow

western blot, immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-DNMT1 Antibody

DNMT1

DNMT1; DNA (cytosine-5-)-methyltransferase 1; CXXC9; DNMT; FLJ16293; MCMT; MGC104992; DNA methyltransferase 1; DNMT1 antibody; DNA (cytosine-5-)-methyltransferase 1 antibody; CXXC9 antibody; DNMT antibody; FLJ16293 antibody; MCMT antibody; MGC104992 antibody; DNA methyltransferase 1 antibody

DNA (cytosine-5)-methyltransferase 1 isoform b; DNA (cytosine-5)-methyltransferase 1; DNA (cytosine-5)-methyltransferase 1; DNA (cytosine-5-)-methyltransferase 1; CXXC-type zinc finger protein 9; DNA methyltransferase HsaI; DNA MTase HsaI; M.HsaI; MCMT

DNMT1

DNMT1; DNMT1; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; m.HsaI; AIM; CXXC9; DNMT; Dnmt1; DNA MTase HsaI; M.HsaI

DNMT1_HUMAN

Polyclonal

Goat

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig

1616

RFESPPKTQPTEDN

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded Human Kidney shows nuclear staining in PCT. Recommended concentration, 3-6ug/ml. Western Blot: Approx 170kDa band observed in lysates of cell line Jurkat (calculated MW of 183kDa according to NP_001370.1). Recommended concentration: 0.5-1.5ug/ml.

Immunogen: Peptide with sequence C-RFESPPKTQPTEDN, from the internal egion of the protein sequence according to NP_001370.1. Epitope: Internal egion

4503351

NP_001370.1

NM_001379.3

144,465 Da

Cysteine And Methionine Metabolism Pathway (104488); Cysteine And Methionine Metabolism Pathway (103421); DNA Methylation Pathway (1269740); Epigenetic Regulation Of Gene Expression Pathway (1269734); Gene Expression Pathway (1269649); Metabolic Pathways (132956); Methionine Degradation Pathway (413353); Methionine Degradation Pathway (468228); MicroRNAs In Cancer Pathway (852705); MicroRNAs In Cancer Pathway (852928)

This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

DNMT1: an ubiquitous DNA methyltransferase that methylates CpG residues. Preferentially methylates hemimethylated DNA. It is responsible for maintaining methylation patterns established in development, and may play an active role in DNA damage repair. Mediates transcriptional repression by direct binding to HDAC2. Its abundance is reduced to non detectable levels at the G0 phase of the cell cycle and is dramatically induced upon entrance into the S-phase of the cell cycle. Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Three isoforms of the human protein produced by alternative splicing have been described. Protein type: EC 2.1.1.37; Methyltransferase, DNA; Cell development/differentiation; Transcription regulation; Amino Acid Metabolism - cysteine and methionine; Methyltransferase. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: cell soma; centric heterochromatin; cytoplasm; nucleoplasm; nucleus; protein complex; replication fork. Molecular Function: DNA (cytosine-5-)-methyltransferase activity; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; DNA binding; DNA-methyltransferase activity; double-stranded DNA binding; estrogen receptor binding; histone deacetylase binding; methyl-CpG binding; protein binding; protein domain specific binding; RNA binding; unmethylated CpG binding; zinc ion binding. Biological Process: aging; brain development; chromatin modification; cytosine methylation within a CG sequence; DNA methylation; gene silencing; maintenance of DNA methylation; negative regulation of gene expression, epigenetic; negative regulation of histone H3-K9 methylation; negative regulation of transcription from RNA polymerase II promoter; neuron differentiation; positive regulation of histone H3-K4 methylation; Ras protein signal transduction; regulation of cell proliferation; response to activity; response to caffeine; response to estradiol stimulus; response to ethanol; response to heat; response to ionizing radiation; response to lipopolysaccharide; response to testosterone stimulus; response to toxin; response to vitamin A; S-adenosylhomocysteine metabolic process; S-adenosylmethioninamine metabolic process; S-adenosylmethionine metabolic process; transcription, DNA-dependent. Disease: Cerebellar Ataxia, Deafness, And Narcolepsy, Autosomal Dominant; Neuropathy, Hereditary Sensory, Type Ie

0.1 mg

300 USD