Goat anti-ABCB5 Antibody | MyBioSource MBS421569 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-ABCB5 Antibody

catalog :

MBS421569

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human

application :

western blot, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS421569

products type :

Antibody

products full name :

Goat anti-ABCB5 Antibody

products short name :

ABCB5

products name syn :

Goat Anti-ABCB5 (aa364-378) Antibody; ATP-binding cassette, sub-family B (MDR/TAP), member 5; ABCB5alpha; ABCB5beta; EST422562; ATP-binding cassette, sub-family B, member 5N: P-glycoprotein ABCB5; ATP-binding cassette; sub-family B (MDR/TAP); member 5 antibody; ABCB5alpha antibody; ABCB5beta antibody; EST422562 antibody; ATP-binding cassette; sub-family B; member 5N: P-glycoprotein ABCB5 antibody

other names :

ATP-binding cassette sub-family B member 5 isoform 2; ATP-binding cassette sub-family B member 5; ATP-binding cassette sub-family B member 5; ATP binding cassette subfamily B member 5; ABCB5 P-gp; P-glycoprotein ABCB5

products gene name :

ABCB5

other gene names :

ABCB5; ABCB5; ABCB5beta; EST422562; ABCB5alpha

uniprot entry name :

ABCB5_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human; Expected from sequence similarity: Human

sequence length :

812

sequence :

QTQHRNTSKKAQ

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 80kDa band observed in lysates of cell line A431(calculated MW of 89.8kDa according to NP_848654.3). Recommended concentration: 0.3-1ug/ml. Flow cytometry: This antibody has been successfully used in FC for Hep3B cells as described in the following paper: Cheung et al, Gastroenterology.2011 Jan;140(1): 344-55, PMID: 20682318. Data obtained from a former batch.

other info1 :

Immunogen: Peptide with sequence C-QTQHRNTSKKAQ, from the internal region of the protein sequence according to NP_848654.3; NP_001157413.1. Epitope: Internal region

other info2 :

Note: This antibody is expected to recognise both reported isoforms (NP_848654.3; NP_001157413.1)

ncbi gi num :

148612844

ncbi acc num :

NP_848654.3

ncbi gb acc num :

NM_178559.5

ncbi mol weight :

89,832 Da

ncbi pathways :

ABC Transporters Pathway (83035); ABC Transporters Pathway (436); ABC-family Proteins Mediated Transport Pathway (1269904); Transmembrane Transport Of Small Molecules Pathway (1269903)

ncbi summary :

ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM, Mar 2008]

uniprot summary :

ABCB5: Plasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Responsible for the resistance to doxorubicin of a subset of malignant melanomas. Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Transporter, ABC family; Membrane protein, integral; Membrane protein, multi-pass; Transporter. Chromosomal Location of Human Ortholog: 7p21.1. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: ATP binding; ATPase activity, coupled to transmembrane movement of substances; efflux transmembrane transporter activity; xenobiotic-transporting ATPase activity. Biological Process: antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent; antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent; cell differentiation; compound eye corneal lens development; metabolic process; multidrug transport; positive regulation of antigen processing and presentation of peptide antigen via MHC class I; regulation of membrane potential; transmembrane transport; xenobiotic transport

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage