catalog number :
MBS421551
products full name :
Goat anti-IGFBP3 Antibody
products short name :
IGFBP3
products name syn :
IGFBP3; insulin-like growth factor binding protein 3; tcag7.703; BP-53; IBP3; IGF-binding protein 3; acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; growth hormone-dependent binding protein; IGFBP3; insulin-like growth factor binding protein 3; tcag7.703; BP-53; IBP3; IGF-binding protein 3; acid stable subunit of the 140 K IGF complex; binding protein 29; binding protein 53; growth hormone-dependent binding protein
other names :
insulin-like growth factor-binding protein 3 isoform a; Insulin-like growth factor-binding protein 3; insulin-like growth factor-binding protein 3; IBP-3; IGFBP-3; binding protein 29; binding protein 53; IGF-binding protein 3; growth hormone-dependent binding protein; acid stable subunit of the 140 K IGF complex; insulin-like growth factor binding protein 3
products gene name :
IGFBP3
other gene names :
IGFBP3; IGFBP3; IBP3; BP-53; IBP3; IBP-3; IGF-binding protein 3; IGFBP-3
uniprot entry name :
IBP3_HUMAN
reactivity :
Human, Mouse, Rat, Dog
sequence :
RYKVDYESQSTDTQN
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB)
app notes :
Western Blot: Approx 28kDa band observed in Human Breast cancer lysates (calculated MW of 31.7kDa according to NP_001013416.1 and 32.2kDa according to NP_000589.2). Recommended concentration: 0.02-0.06ug/ml.
An additional band of 50kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot. Peptide ELISA: antibody detection limit dilution 1:64000.
other info1 :
Epitope: Internal Region. Immunogen: Peptide with sequence C-RYKVDYESQSTDTQN, from the internal region of the protein sequence according to NP_001013416.1; NP_000589.2. Corresponding Immunizing Peptide: Catalog #MBS426446
other info2 :
Important Note: This antibody is expected to recognise both reported isoforms (NP_001013416.1 and NP_000589.2).
ncbi acc num :
NP_001013416.1
ncbi gb acc num :
NM_001013398.1
ncbi mol weight :
32,223 Da
ncbi pathways :
Direct P53 Effectors Pathway 137939!!Integrated Pancreatic Cancer Pathway 711360!!Metabolism Of Proteins Pathway 106230!!Myometrial Relaxation And Contraction Pathways 198759!!Regulation Of Insulin-like Growth Factor (IGF) Transport And Uptake By Insulin-like Growth Factor Binding Proteins (IGFBPs) Pathway 105908!!Senescence And Autophagy Pathway 198780!!Transcriptional Misregulation In Cancer Pathway 523016!!Transcriptional Misregulation In Cancer Pathway 522987!!P53 Signaling Pathway 83055!!P53 Signaling Pathway 465
ncbi summary :
This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]