Goat anti-DIO2 Antibody | MyBioSource MBS421525 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-DIO2 Antibody

catalog :

MBS421525

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS421525

products type :

Antibody

products full name :

Goat anti-DIO2 Antibody

products short name :

DIO2

products name syn :

DIO2; deiodinase, iodothyronine, type II; 5DII; D2; SelY; TXDI2; thyroxine deiodinase, type II; type 2 iodothyronine deiodinase; type II iodothyronine deiodinaseN: type-II 5'deiodinase; DIO2 antibody; deiodinase; iodothyronine; type II antibody; 5DII antibody; D2 antibody; SelY antibody; TXDI2 antibody; thyroxine deiodinase; type II antibody; type 2 iodothyronine deiodinase antibody; type II iodothyronine deiodinaseN: type-II 5 1deiodinase antibody

other names :

type II iodothyronine deiodinase isoform a; Type II iodothyronine deiodinase; type II iodothyronine deiodinase; deiodinase, iodothyronine, type II; 5DII; DIOII; Type 2 DI; Type-II 5'-deiodinase

products gene name :

DIO2

other gene names :

DIO2; DIO2; D2; 5DII; SelY; DIOII; TXDI2; ITDI2; TXDI2

uniprot entry name :

IOD2_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat, Pig

sequence length :

273

sequence :

EVKKHQNQEDRC

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Immunohistochemistry (IHC)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 128000. Immunohistochemistry: Cryo-sectioned Mouse Brain (Hippocampus) shows staining in the granular layer of the dentate gyrus. Recommended concentration, 2-3ug/ml. Data obtained from anonymous customer. Western Blot: Preliminary experiments gave an approx 65-70kDa band in Human Heart and Thyroid lysates after 0. 05ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.5kDa according to NP_NP_000784.2 and of 34.6kDa according to NP_001007024.1. The 65-70kDa band was successfully blocked by incubation with the immunizing peptide.

other info1 :

Immunogen: Peptide with sequence EVKKHQNQEDRC, from the internal region of the protein sequence according to NP_000784.2; NP_054644.1; NP_001007024.1. Epitope: Internal region

other info2 :

Note: This antibody is expected to recognise both reported isoform a (NP_000784.2 and NP_054644.1) and isoform b (NP_001007024.1).

ncbi gi num :

7549805

ncbi acc num :

NP_000784.2

ncbi gb acc num :

NM_000793.5

ncbi mol weight :

34,704 Da

ncbi pathways :

Amine-derived Hormones Pathway (1270175); Metabolism Pathway (1269956); Metabolism Of Amino Acids And Derivatives Pathway (1270158); Regulation Of Thyroid Hormone Activity Pathway (1270178); Selenium Metabolism And Selenoproteins Pathway (198846); Selenium Pathway (198825); Thyroid Hormone Signaling Pathway (946598); Thyroxine Biosynthesis Pathway (1270177); Thyroid Hormone Metabolism I (via Deiodination) Pathway (545360); Thyroid Hormone Metabolism I (via Deiodination) Pathway (139512)

ncbi summary :

The protein encoded by this gene belongs to the iodothyronine deiodinase family. It catalyzes the conversion of prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4) to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by outer ring 5'-deiodination. This gene is widely expressed, including in thyroid, placenta, pituitary and brain. It is thought to be responsible for the 'local' production of T3, and thus important in influencing thyroid hormone action in these tissues. It has also been reported to be highly expressed in thyroids of patients with Graves disease, and in follicular adenomas. The intrathyroidal T4 to T3 conversion by this enzyme may contribute significantly to the relative increase in thyroidal T3 production in these patients. This protein is a selenoprotein containing the rare selenocysteine (Sec) amino acid at its active site, and may contain additional Sec residues. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2016]

uniprot summary :

DIO2: Responsible for the deiodination of T4 (3,5,3',5'- tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development. Belongs to the iodothyronine deiodinase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Oxidoreductase; EC 1.97.1.10; Membrane protein, integral. Chromosomal Location of Human Ortholog: 14q24.2-q24.3. Cellular Component: integral to membrane; membrane; plasma membrane. Molecular Function: selenium binding; thyroxine 5'-deiodinase activity; ubiquitin protein ligase binding. Biological Process: hormone biosynthetic process; selenocysteine incorporation; thyroid hormone generation; thyroid hormone metabolic process

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage