catalog number :
MBS421519
products full name :
Goat anti-CMG1 / CCDC2 / IFT74 Antibody
products short name :
CMG1/CCDC2/IFT74
products name syn :
IFT74; CCDC2; CMG-1; FLJ22621; MGC111562; intraflagellar transport 74 homolog (Chlamydomonas); capillary morphogenesis protein 1; coiled-coil domain containing 2; CMG1; IFT74; CCDC2; CMG-1; FLJ22621; MGC111562; intraflagellar transport 74 homolog (Chlamydomonas); capillary morphogenesis protein 1; coiled-coil domain containing 2; CMG1
other names :
intraflagellar transport protein 74 homolog isoform a; Intraflagellar transport protein 74 homolog; intraflagellar transport protein 74 homolog; coiled-coil domain containing 2; capillary morphogenesis protein 1; intraflagellar transport 74 homolog; capillary morphogenesis gene 1 protein; coiled-coil domain-containing protein 2; intraflagellar transport 74; Capillary morphogenesis gene 1 protein; CMG-1; Coiled-coil domain-containing protein 2
products gene name :
IFT74
other gene names :
IFT74; IFT74; CMG1; CCDC2; CMG-1; CCDC2; CMG1; CMG-1
uniprot entry name :
IFT74_HUMAN
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
app notes :
Immunohistochemistry: In paraffin embedded Human Liver shows vesicular staining in cytoplasm of hepatocytes. Recommended concentration, 5-10ug/ml. Western Blot: Approx 70-75kDa band observed in lysates of cell line HEK293 (calculated MW of 69.2kDa according to AAK77221.1, but 51.4kDa according to NP_079379.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Momeni et al, BMC Neurol. 2006 Dec 13;6:44.; PMID: 17166276). Recommended concentration: 0.3-1ug/ml. Peptide ELISA: antibody detection limit dilution 1:16000. Immunofluorescence: Was successfully used at 2.5mg/ml to stain rat cortical neurons (see picture and PMID: 17166276).
other info1 :
Epitope: C Terminus. Immunogen: Peptide with sequence C-KTIVDALHSTSGN, from the C Terminus of the protein sequence according to NP_001092692.1; NP_001092693.1; NP_079379.2; AAK77221.1.
other info2 :
Corresponding Immunizing Peptide: Catalog #MBS425246
ncbi acc num :
NP_001092692.1
ncbi gb acc num :
NM_001099222.1
ncbi mol weight :
42,332 Da