product summary
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company name :
MyBioSource
product type :
antibody
product name :
Goat anti-NCAM2/OCAM Antibody
catalog :
MBS421506
quantity :
0.1 mg
price :
305 USD
clonality :
polyclonal
host :
goat
conjugate :
nonconjugated
reactivity :
human, mouse, rat, dog, cow
application :
immunohistochemistry, enzyme immunoassay
more info or order :
image
image 1 :
(0.5ug/ml) 24h-staining of PFA-perfused cryosection of Mouse Olfactory bub. Antigen retrieval with methanol (-20C, 10min) followed by 1% SDS(10min), IF-staning. Data obtained from anonymous customer.
image 2 :
(0.5ug/ml) 48h-staining of PFA-perfused cryosection of Mouse Olfactory bulb. Antigen retrieval with citrate buffer pH 6 at 95C for 10min, IF-staning for NCAM2 (red), VGLUT2 (blue) and NQO1 (green), data obtained from anonymus customer.
image 3 :
(3.75 ug/mL) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH, AP-staining.
product information
catalog number :
MBS421506
products type :
Antibody
products full name :
Goat anti-NCAM2/OCAM Antibody
products short name :
[NCAM2/OCAM]
products name syn :
[NCAM2; neural cell adhesion molecule 2; HGNC: 7657; MGC51008; NCAM21; OCAM; NCAM2 antibody; neural cell adhesion molecule 2 antibody; HGNC: 7657 antibody; MGC51008 antibody; NCAM21 antibody; OCAM antibody; NCAM2 / OCAM]
other names :
[neural cell adhesion molecule 2; Neural cell adhesion molecule 2; neural cell adhesion molecule 2; neural cell adhesion molecule 2]
products gene name :
[NCAM2]
products gene name syn :
[OCAM]
other gene names :
[NCAM2; NCAM2; NCAM21; NCAM21; N-CAM-2; NCAM-2]
uniprot entry name :
NCAM2_HUMAN
clonality :
Polyclonal
host :
Goat
reactivity :
Tested: Human, Mouse. Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
sequence length :
837
sequence :
KVSNDIIQSKEDDSK
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
storage stability :
Aliquot and store at -20°C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Immunohistochemistry (IHC)
app notes :
Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Preliminary experiments gave an approx 150 and 28kDa bands in Human and Mouse Brain lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 93. 0kDa according to NP_004531.2. Both observed bands were successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: Frozen section of Olfactory bulb from transcardially PFA-perfused Mouse shows strong staining of axon bundles (right side middle, figure 1) and moderate peripheral staining outside the glomeruli (top, figure 1). NCAM2 (red) is stained complementary to VGLUT2 (blue) and NQO1 (green) in figure 2. Recommended concentration:0.5 ug/mL. Paraffin embedded Human Brain (Cortex). Recommended concentration is: 3.75ug/ml.
image1 heading :
Immunofluorescence (IF)
image2 heading :
Immunofluorescence (IF)
image3 heading :
Testing Data(TD)
other info1 :
Immunogen: Peptide with sequence C-KVSNDIIQSKEDDSK, from the C Terminus of the protein sequence according to NP_004531.2. Epitope: C Terminus
ncbi gi num :
33519481
ncbi acc num :
NP_004531.2
ncbi gb acc num :
NM_004540.3
ncbi pathways :
Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Prion Diseases Pathway (101144); Prion Diseases Pathway (100065)
ncbi summary :
The protein encoded by this gene belongs to the immunoglobulin superfamily. It is a type I membrane protein and may function in selective fasciculation and zone-to-zone projection of the primary olfactory axons. [provided by RefSeq, Jul 2008]
uniprot summary :
NCAM2: May play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons. Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion. Chromosomal Location of Human Ortholog: 21q21.1. Cellular Component: axon; integral to membrane; plasma membrane. Biological Process: neuron adhesion
size1 :
0.1 mg
price1 :
305 USD
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
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