Goat anti-AIF1/IBA1 (isoform 3) Antibody | MyBioSource MBS421481 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-AIF1/IBA1 (isoform 3) Antibody

catalog :

MBS421481

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog

application :

western blot, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS421481

products type :

Antibody

products full name :

Goat anti-AIF1/IBA1 (isoform 3) Antibody

products short name :

AIF1/IBA1

products name syn :

Goat Anti-AIF1 / IBA1 (isoforms 1 + 3) Antibody; AIF1; IBA1; allograft inflammatory factor 1; DASS-82G15.2; AIF-1; IRT-1; Em: AF129756.17; interferon gamma responsive transcript; ionized calcium-binding adapter molecule; AIF1 antibody; IBA1 antibody; allograft inflammatory factor 1 antibody; DASS-82G15.2 antibody; AIF-1 antibody; IRT-1 antibody; Em: AF129756.17 antibody; interferon gamma responsive transcript antibody; ionized calcium-binding adapter molecule antibody; AIF1 / IBA1 (isoform 3)

other names :

allograft inflammatory factor 1 isoform 3; Allograft inflammatory factor 1; allograft inflammatory factor 1; allograft inflammatory factor 1; Ionized calcium-binding adapter molecule 1; Protein G1

products gene name :

AIF1

products gene name syn :

AIF1; IBA1

other gene names :

AIF1; AIF1; IBA1; IRT1; AIF-1; IRT-1; G1; IBA1; AIF-1

uniprot entry name :

AIF1_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog

sequence length :

147

sequence :

NKQFLDDPKYSSDED

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded Mouse Brain shows staining of astrocytes. Recommended concentration, 2-4ug/ml. Western Blot: Approx 17kDa band observed in Human Brain (Frontal Cortex) and Peripheral Blood Lymphocytes lysates (calculated MW of 16.7kDa according to NP_001614.3). Recommended concentration: 0.5-1.5ug/ml.

other info1 :

Immunogen: Peptide with sequence C-NKQFLDDPKYSSDED, from the internal region of the protein sequence according to NP_001614.3. Epitope: Internal region

other info2 :

Note: This antibody is expected to recognise isoform 3 (NP_001614.3) only.

ncbi gi num :

14574568

ncbi acc num :

NP_001614.3

ncbi gb acc num :

NM_001623.4

ncbi mol weight :

14,617 Da

ncbi pathways :

Spinal Cord Injury Pathway (739007)

ncbi summary :

This gene encodes a protein that binds actin and calcium. This gene is induced by cytokines and interferon and may promote macrophage activation and growth of vascular smooth muscle cells and T-lymphocytes. Polymorphisms in this gene may be associated with systemic sclerosis. Alternative splicing results in multiple transcript variants, but the full-length and coding nature of some of these variants is not certain. [provided by RefSeq, Jan 2016]

uniprot summary :

AIF1: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: actin filament; cytoplasm; cytosol; lamellipodium; nucleus; perikaryon; perinuclear region of cytoplasm; phagocytic cup. Molecular Function: actin filament binding; calcium ion binding. Biological Process: actin filament bundle formation; actin filament polymerization; cellular response to extracellular stimulus; cellular response to hormone stimulus; inflammatory response; microglial cell activation; negative regulation of apoptosis; negative regulation of smooth muscle cell proliferation; phagocytosis, engulfment; positive regulation of muscle hyperplasia; positive regulation of nitric oxide biosynthetic process; positive regulation of protein amino acid phosphorylation; positive regulation of smooth muscle cell proliferation; positive regulation of T cell proliferation; Rac protein signal transduction; response to axon injury; response to electrical stimulus; response to glucocorticoid stimulus

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage