catalog number :
MBS421449
products full name :
Goat anti-Radixin Antibody
products short name :
Radixin
products name syn :
RDX; radixin; RDX; radixin
other names :
radixin isoform 2; Radixin; radixin; radixin
other gene names :
RDX; RDX; DFNB24
uniprot entry name :
RADI_HUMAN
reactivity :
Human, Mouse, Rat, Dog
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB)
app notes :
Western Blot: Approx 80kDa band observed in Human Lymph Node, Tonsil and Spleen lysates (calculated MW of 68.6kDa according to NP_002897.1). The same 80kDa was also observed in lysates of several Mouse and Rat tissues and in lysates of cell lines NIH3T3, HeLa, Jurkat, and A431. The observed molecular weight corresponds to earlier findings in literature (Magendantz et al, J Biol Chem. 1995 Oct 27;270(43):25324-7. PMID: 7592691). Recommended concentration: 0.01-0.03ug/ml. Peptide ELISA: antibody detection limit dilution 1:4000.
other info1 :
Epitope: Internal Region. Immunogen: Peptide with sequence C-QARDETKKTQND, from the internal region of the protein sequence according to NP_002897.1.
other info2 :
Corresponding Immunizing Peptide: Catalog #MBS425507
ncbi acc num :
NP_002897.1
ncbi gb acc num :
NM_002906.3
ncbi mol weight :
71,049 Da
ncbi pathways :
Axon Guidance Pathway 105688!!Developmental Biology Pathway 477129!!L1CAM Interactions Pathway 161003!!MicroRNAs In Cancer Pathway 852705!!MicroRNAs In Cancer Pathway 852928!!Proteoglycans In Cancer Pathway 782000!!Proteoglycans In Cancer Pathway 782054!!Recycling Pathway Of L1 161004!!Regulation Of Actin Cytoskeleton Pathway 198874!!Regulation Of Actin Cytoskeleton Pathway 83089
ncbi summary :
Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. It is highly similar in sequence to both ezrin and moesin. The radixin gene has been localized by fluorescence in situ hybridization to 11q23. A truncated version representing a pseudogene (RDXP2) was assigned to Xp21.3. Another pseudogene that seemed to lack introns (RDXP1) was mapped to 11p by Southern and PCR analyses. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]