Goat anti-14-3-3 sigma/Stratifin Antibody | MyBioSource MBS421444 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-14-3-3 sigma/Stratifin Antibody

catalog :

MBS421444

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog

application :

western blot, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS421444

products type :

Antibody

products full name :

Goat anti-14-3-3 sigma/Stratifin Antibody

products short name :

14-3-3 sigma/Stratifin

products name syn :

SFN; 14-3-3sigma; stratifin; HGNC: 10773; YWHAS; 14-3-3sigma antibody; stratifin antibody; HGNC: 10773 antibody; SFN antibody; YWHAS antibody; 14-3-3 sigma / Stratifin

other names :

14-3-3 protein sigma; 14-3-3 protein sigma; 14-3-3 protein sigma; stratifin; Epithelial cell marker protein 1; Stratifin

products gene name :

SFN

products gene name syn :

14-3-3 sigma; Stratifin

other gene names :

SFN; SFN; YWHAS; HME1

uniprot entry name :

1433S_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

sequence length :

248

sequence :

DLHTLSEDSYKDST

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 16000. Immunohistochemistry: In paraffin embedded Human Skin shows strong nuclear staining in selected cells of the basal layer. Recommended concentration, 2-4ug/ml. Western Blot: Approx.28kDa band observed in Human Skin lysates (calculated MW of 27.8kDa according to NP_006133.1). Recommended concentration: 0. 03-0.1ug/ml.

other info1 :

Immunogen: Peptide with sequence C-DLHTLSEDSYKDST, from the internal region of the protein sequence according to NP_006133.1. Epitope: Internal region

ncbi gi num :

5454052

ncbi acc num :

NP_006133.1

ncbi gb acc num :

NM_006142.3

ncbi mol weight :

24,336 Da

ncbi pathways :

Activation Of BAD And Translocation To Mitochondria Pathway (1270271); Activation Of BH3-only Proteins Pathway (1270270); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Alpha6-Beta4 Integrin Signaling Pathway (198807); Apoptosis Pathway (1270262); Calcium Regulation In The Cardiac Cell Pathway (198906); Cell Cycle Pathway (1269741); Cell Cycle Checkpoints Pathway (1269742); Cell Cycle Pathway (83054)

uniprot summary :

14-3-3 sigma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. Homodimer. Interacts with KRT17 and SAMSN1. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with GAB2. Interacts with SRPK2. Present mainly in tissues enriched in stratified squamous keratinizing epithelium. Belongs to the 14-3-3 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 1p36.11. Cellular Component: cytoplasm; cytoplasmic vesicle membrane; cytosol; extracellular space; mitochondrion; nucleus. Molecular Function: phosphoprotein binding; protein binding; protein domain specific binding; protein kinase binding; protein kinase C inhibitor activity. Biological Process: DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA damage response, signal transduction resulting in induction of apoptosis; keratinization; negative regulation of caspase activity; negative regulation of protein kinase activity; positive regulation of cell growth; positive regulation of epidermal cell differentiation; positive regulation of protein export from nucleus; regulation of cyclin-dependent protein kinase activity; regulation of epidermal cell division; release of cytochrome c from mitochondria; signal transduction

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage