Goat anti-PGAM1/PGAM2/PGAM4 Antibody | MyBioSource MBS421435 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-PGAM1/PGAM2/PGAM4 Antibody

catalog :

MBS421435

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, cow

application :

western blot, enzyme immunoassay

more info or order :

MyBioSource product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; cow; 1:500	Janjanam J, Singh S, Jena M, Varshney N, Kola S, Kumar S, et al. Comparative 2D-DIGE proteomic analysis of bovine mammary epithelial cells during lactation reveals protein signatures for lactation persistency and milk yield. PLoS ONE. 2014;9:e102515 pubmed publisher

product information

catalog number :

MBS421435

products type :

Antibody

products full name :

Goat anti-PGAM1/PGAM2/PGAM4 Antibody

products short name :

PGAM1/PGAM2/PGAM4

products name syn :

PGAM1; PGAM2; phosphoglycerate mutase 1 (brain); PGAMA; RP11-452K12.8; OTTHUMP00000059414; Phosphoglycerate mutase A, nonmuscle form; phosphoglycerate mutase 2 (muscle); MGC88743; PGAMM; Phosphoglycerate mutase, muscle form; PGAM3; phosphoglycerate mutas; PGAM1 antibody; PGAM2 antibody; phosphoglycerate mutase 1 (brain) antibody; PGAMA antibody; RP11-452K12.8 antibody; OTTHUMP00000059414 antibody; Phosphoglycerate mutase A; nonmuscle form antibody; phosphoglycerate mutase 2 (muscle) antibody; MGC88743 antibody; PGAMM antibody; Phosphoglycerate mutase; muscle form antibody; PGAM3 antibody; phosphoglycerate mutase family member 4 antibody; PGAM-B antibody; dJ1000K24.1 antibody; phosphoglycerate mutase family 3 antibody; phosphoglycerate mutase family 4 antibody; phosphoglycerate mutase processed protein antibody; bisphosphoglycerate mutase 1 antibody; bisphosphoglycerate mutase 2 antibody; bisphosphoglycerate mutase 4 antibody; BPG-dependent PGAM 1 antibody; OTTHUMP00000020190 antibody; PGAM-M antibody; BPG-dependent PGAM 2 antibody; OTTHUMP00000063546 antibody; PGAM1 / PGAM2 / PGAM4

other names :

phosphoglycerate mutase 1 isoform 1; Phosphoglycerate mutase 1; phosphoglycerate mutase 1; phosphoglycerate mutase 1; BPG-dependent PGAM 1; Phosphoglycerate mutase isozyme B; PGAM-B

products gene name :

PGAM1

products gene name syn :

PGAM2; PGAM4

other gene names :

PGAM1; PGAM1; PGAMA; PGAM-B; HEL-S-35; PGAMA; PGAM-B

uniprot entry name :

PGAM1_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

sequence length :

254

sequence :

KAMEAVAAQGKAKK

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 27kDa band observed in Human Liver and Human Brain lysates (calculated MW of 28.8kDa according to NP_002620.1, NP_000281.2, NP_001025062.1). Recommended concentration: 0.01-0.03ug/ml.

other info1 :

Immunogen: Peptide with sequence C-KAMEAVAAQGKAKK, from the C Terminus of the protein sequence according to NP_002620.1; NP_000281.2; NP_001025062.1. Epitope: C Terminus

other info2 :

Note: Please note this antibody is expected to recognize the products of 3 highly similar genes.

ncbi gi num :

4505753

ncbi acc num :

NP_002620.1

ncbi gb acc num :

NM_002629.3

ncbi mol weight :

28,804 Da

ncbi pathways :

Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Central Carbon Metabolism In Cancer Pathway (1059538); Central Carbon Metabolism In Cancer Pathway (1084231); Glucagon Signaling Pathway (1144996); Glucagon Signaling Pathway (1158201); Gluconeogenesis Pathway (1269961); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (413342)

ncbi summary :

The protein encoded by this gene is a mutase that catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

uniprot summary :

PGAM1: Interconversion of 3- and 2-phosphoglycerate with 2,3- bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. Homodimer. In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues. Belongs to the phosphoglycerate mutase family. BPG- dependent PGAM subfamily. Protein type: EC 5.4.2.4; EC 3.1.3.13; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Isomerase; Phosphatase (non-protein); EC 5.4.2.11. Chromosomal Location of Human Ortholog: 10q25.3. Cellular Component: cytoplasm; cytosol; membrane; myelin sheath. Molecular Function: 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase activity; bisphosphoglycerate mutase activity; bisphosphoglycerate phosphatase activity; phosphoglycerate mutase activity; protein binding; protein kinase binding. Biological Process: dephosphorylation; gluconeogenesis; glycolysis; regulation of glycolysis; regulation of pentose-phosphate shunt; respiratory burst

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage