Goat anti-ENPP1/PC1 Antibody | MyBioSource MBS421434 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-ENPP1/PC1 Antibody

catalog :

MBS421434

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS421434

products type :

Antibody

products full name :

Goat anti-ENPP1/PC1 Antibody

products short name :

ENPP1/PC1

products name syn :

ENPP1; PC-1; ectonucleotide pyrophosphatase/phosphodiesterase 1; HGNC: 3356; M6S1; NPP1; NPPS; PCA1; PDNP1; Ly-41 antigen; OTTHUMP00000043194; alkaline phosphodiesterase 1; membrane component, chromosome 6, surface marker 1; phosphodiesterase I/nucleotide; ENPP1 antibody; PC-1 antibody; ectonucleotide pyrophosphatase/phosphodiesterase 1 antibody; HGNC: 3356 antibody; M6S1 antibody; NPP1 antibody; NPPS antibody; PCA1 antibody; PDNP1 antibody; Ly-41 antigen antibody; OTTHUMP00000043194 antibody; alkaline phosphodiesterase 1 antibody; membrane component; chromosome 6; surface marker 1 antibody; phosphodiesterase I/nucleotide pyrophosphatase 1 antibody; plasma-cell membrane glycoprotein 1 antibody; ENPP1 / PC1

other names :

ectonucleotide pyrophosphatase/phosphodiesterase family member 1; Ectonucleotide pyrophosphatase/phosphodiesterase family member 1; ectonucleotide pyrophosphatase/phosphodiesterase family member 1; ectonucleotide pyrophosphatase/phosphodiesterase 1; Membrane component chromosome 6 surface marker 1; Phosphodiesterase I/nucleotide pyrophosphatase 1; Plasma-cell membrane glycoprotein PC-1Including the following 2 domains:Alkaline phosphodiesterase I (EC:3.1.4.1)Nucleotide pyrophosphatase (EC:3.6.1.9); NPPase

products gene name :

ENPP1

products gene name syn :

PC1

other gene names :

ENPP1; ENPP1; M6S1; NPP1; NPPS; PC-1; PCA1; ARHR2; COLED; PDNP1; M6S1; NPPS; PC1; PDNP1; E-NPP 1; NPPase

uniprot entry name :

ENPP1_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat

sequence length :

925

sequence :

KTHLPTFSQED

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB-Trf), Immunohistochemistry (IHC)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 128000. Immunohistochemistry: In paraffin embedded Human Liver shows membranous staining in many hepatocytes. Recommended concentration, 2-4ug/ml. Western Blot: In transfected HEK293 transiently expressing ENPP1 a band of approx.100 kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 105kDa according to NP_006199.2. Data obtained from previous batch (different goat). Recommended concentration, 1-3ug/ml.

other info1 :

Immunogen: Peptide with sequence C-KTHLPTFSQED, from the C Terminus of the protein sequence according to NP_006199.2. Epitope: C Terminus

ncbi gi num :

170650661

ncbi acc num :

NP_006199.2

ncbi gb acc num :

NM_006208.2

ncbi mol weight :

104,924 Da

ncbi pathways :

Endochondral Ossification Pathway (198812); Insulin Signaling Pathway (198845); Metabolic Pathways (132956); Metabolism Pathway (1269956); Metabolism Of Vitamins And Cofactors Pathway (1270144); Metabolism Of Water-soluble Vitamins And Cofactors Pathway (1270145); Nicotinate And Nicotinamide Metabolism Pathway (83014); Nicotinate And Nicotinamide Metabolism Pathway (400); Pantothenate And CoA Biosynthesis Pathway (83015); Pantothenate And CoA Biosynthesis Pathway (401)

ncbi summary :

This gene is a member of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. The encoded protein is a type II transmembrane glycoprotein comprising two identical disulfide-bonded subunits. This protein has broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. This protein may function to hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and may also hydrolyze diadenosine polyphosphates. Mutations in this gene have been associated with 'idiopathic' infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance. [provided by RefSeq, Jul 2008]

uniprot summary :

ENPP1: Involved primarily in ATP hydrolysis at the plasma membrane. Plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. In vitro, has a broad specificity, hydrolyzing other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity. Homodimer; disulfide-linked. Interacts with INSR. Expressed in plasma cells and also in a number of non-lymphoid tissues, including the distal convoluted tubule of the kidney, chondrocytes and epididymis. At low concentrations of ATP, a phosphorylated intermediate is formed which inhibits further hydrolysis. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family. Protein type: EC 3.1.4.1; EC 3.6.1.9; Phosphodiesterase; Motility/polarity/chemotaxis; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Carbohydrate Metabolism - starch and sucrose; Cofactor and Vitamin Metabolism - riboflavin; Nucleotide Metabolism - purine; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Membrane protein, integral; Phosphatase (non-protein). Chromosomal Location of Human Ortholog: 6q22-q23. Cellular Component: basolateral plasma membrane; cell surface; extracellular space; integral to membrane; integral to plasma membrane; lysosomal membrane; plasma membrane. Molecular Function: 3'-phosphoadenosine 5'-phosphosulfate binding; ATP binding; calcium ion binding; insulin receptor binding; nucleic acid binding; nucleoside-triphosphate diphosphatase activity; nucleotide diphosphatase activity; phosphodiesterase I activity; polysaccharide binding; protein binding; protein homodimerization activity; scavenger receptor activity; zinc ion binding. Biological Process: 3'-phosphoadenosine 5'-phosphosulfate metabolic process; biomineral formation; cellular phosphate ion homeostasis; cellular response to insulin stimulus; generation of precursor metabolites and energy; immune response; inorganic diphosphate transport; negative regulation of cell growth; negative regulation of fat cell differentiation; negative regulation of glucose import; negative regulation of glycogen biosynthetic process; negative regulation of insulin receptor signaling pathway; negative regulation of protein amino acid autophosphorylation; nucleoside triphosphate catabolic process; phosphate metabolic process; receptor-mediated endocytosis; regulation of bone mineralization; riboflavin metabolic process; sequestering of triacylglycerol. Disease: Arterial Calcification, Generalized, Of Infancy, 1; Cole Disease; Diabetes Mellitus, Noninsulin-dependent; Hypophosphatemic Rickets, Autosomal Recessive, 2; Obesity

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage