Goat anti-CIAS1 / Cryopyrin (Internal) Antibody | MyBioSource MBS421430 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-CIAS1 / Cryopyrin (Internal) Antibody

catalog :

MBS421430

quantity :

0.1 mg

price :

290 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, cow

application :

western blot, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS421430

products type :

Antibody

products full name :

Goat anti-CIAS1 / Cryopyrin (Internal) Antibody

products short name :

CIAS1/Cryopyrin

products name syn :

CIAS1; cold autoinflammatory syndrome 1; AGTAVPRL; AII/AVP; C1orf7; FCAS; FCU; MWS; NALP3; PYPAF1; AII/AVP receptor-like; Muckle-Wells syndrome; NACHT; LRR and PYD containing protein 3; PYRIN-containing APAF1-like protein 1; angiotensin/vasopressin receptor AII/AVP-like; cryopyrin; Goat Anti-Cryopirin / NALP3 / NLRP3; CIAS1; cold autoinflammatory syndrome 1; AGTAVPRL; AII/AVP; C1orf7; FCAS; FCU; MWS; NALP3; PYPAF1; AII/AVP receptor-like; Muckle-Wells syndrome; NACHT, LRR and PYD containing protein 3; PYRIN-containing APAF1-like protein 1; angiotensin/vasopressin receptor AII/AVP-like; cryopyrin

other names :

NACHT, LRR and PYD domains-containing protein 3 isoform a; NACHT, LRR and PYD domains-containing protein 3; NACHT, LRR and PYD domains-containing protein 3; cryopyrin; caterpiller protein 1.1; PYRIN-containing APAF1-like protein 1; NACHT, LRR and PYD containing protein 3; cold autoinflammatory syndrome 1 protein; NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3; NLR family, pyrin domain containing 3; Angiotensin/vasopressin receptor AII/AVP-like; Caterpiller protein 1.1; CLR1.1; Cold autoinflammatory syndrome 1 protein; Cryopyrin; PYRIN-containing APAF1-like protein 1

products gene name :

NLRP3

other gene names :

NLRP3; NLRP3; AII; AVP; FCU; MWS; FCAS; CIAS1; FCAS1; NALP3; C1orf7; CLR1.1; PYPAF1; AGTAVPRL; C1orf7; CIAS1; NALP3; PYPAF1; CLR1.1

uniprot entry name :

NALP3_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Human, Mouse, Rat, Dog, Pig, Cow

sequence length :

1036

sequence :

DLYEKAKRDEPK

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)

app notes :

Immunohistochemistry: In paraffin embedded Human Tonsil shows focal and interstitial staining of the inflammatory elements close to the epithelia of the tonsil crypts. Recommended concentration, 2-4ug/ml. Western Blot: Preliminary experiments gave an approx 70kDa band in human lymph node and tonsil lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 118kDa according to NP_004886.3 and 106kDa according to NP_899632.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide. Peptide ELISA: antibody detection limit dilution 1:64000.

other info1 :

Epitope: Internal Region. Immunogen: Peptide with sequence C-DLYEKAKRDEPK, from the internal region of the protein sequence according to NP_004886.3; NP_899632.1; NP_001230062.1; NP_001120933.1; NP_001120934.1. Corresponding Immunizing Peptide: Catalog #MBS428332

other info2 :

Important Note: This antibody is expected to recognise all five reported isoforms (NP_004886.3; NP_899632.1; NP_001230062.1; NP_001120933.1; NP_001120934.1). Reported variants represent identical protein (NP_004886.3 and NP_001073289.1).

ncbi gi num :

34878693

ncbi acc num :

NP_004886.3

ncbi gb acc num :

NM_004895.4

ncbi mol weight :

115,968 Da

ncbi pathways :

Immune System Pathway 106386!!Inflammasomes Pathway 366166!!Influenza A Pathway 217173!!Influenza A Pathway 217150!!Innate Immune System Pathway 106387!!NOD Pathway 198792!!NOD-like Receptor Signaling Pathway 122191!!NOD-like Receptor Signaling Pathway 122131!!Nucleotide-binding Domain, Leucine Rich Repeat Containing Receptor (NLR) Signaling Pathways 366164!!Pertussis Pathway 218111

ncbi summary :

This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. [provided by RefSeq, Oct 2008]

size :

0.1 mg

price :

290 USD

more info or order :

MyBioSource product webpage