Goat anti-NCF4/P40PHOX Antibody | MyBioSource MBS421423 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Goat anti-NCF4/P40PHOX Antibody

catalog :

MBS421423

quantity :

0.1 mg

price :

300 USD

clonality :

polyclonal

host :

goat

conjugate :

nonconjugated

reactivity :

human, dog

application :

western blot, immunohistochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS421423

products type :

Antibody

products full name :

Goat anti-NCF4/P40PHOX Antibody

products short name :

NCF4/P40PHOX

products name syn :

NCF4; P40PHOX; neutrophil cytosolic factor 4, 40kDa; CTA-833B7.1; MGC3810; NCF; SH3PXD4; OTTHUMP00000028736; OTTHUMP00000043756; neutrophil NADPH oxidase factor 4; neutrophil cytosol factor 4; neutrophil cytosolic factor 4 (40kD); NCF4 antibody; P40PHOX antibody; neutrophil cytosolic factor 4; 40kDa antibody; CTA-833B7.1 antibody; MGC3810 antibody; NCF antibody; SH3PXD4 antibody; OTTHUMP00000028736 antibody; OTTHUMP00000043756 antibody; neutrophil NADPH oxidase factor 4 antibody; neutrophil cytosol factor 4 antibody; neutrophil cytosolic factor 4 (40kD) antibody; NCF4 / P40PHOX

other names :

neutrophil cytosol factor 4 isoform 1; Neutrophil cytosol factor 4; neutrophil cytosol factor 4; neutrophil cytosolic factor 4; Neutrophil NADPH oxidase factor 4; SH3 and PX domain-containing protein 4; p40-phox; p40phox

products gene name :

NCF4

products gene name syn :

P40PHOX

other gene names :

NCF4; NCF4; NCF; P40PHOX; SH3PXD4; SH3PXD4; NCF-4; p40phox

uniprot entry name :

NCF4_HUMAN

clonality :

Polyclonal

host :

Goat

reactivity :

Tested: Human; Expected from sequence similarity: Human, Dog

sequence length :

339

sequence :

KDFPEEDDPTN

purity :

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

form :

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

concentration :

100ug specific antibody in 200ul

storage stability :

Aliquot and store at -20 degree C. Minimize freezing and thawing.

tested application :

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded Human Tonsil shows staining of the cytoplasm in some cells. Recommended concentration, 2-4ug/ml. Western Blot: Approx.38kDa band observed in lysates of cell line Daudi (calculated MW of 39. 0kDa according to NP_000622.2 and 38.8kDa according to NP_038202.1). In transfected HEK293 transiently expressing P40PHOX a band of approx. 42kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml.

other info1 :

Immunogen: Peptide with sequence C-KDFPEEDDPTN, from the internal region of the protein sequence according to NP_000622.2; NP_038202.1. Epitope: Internal region

other info2 :

Note: This antibody is expected to recognise both reported isoforms (NP_000622.2 and NP_038202.1).

ncbi gi num :

47519798

ncbi acc num :

NP_000622.2

ncbi gb acc num :

NM_000631.4

ncbi mol weight :

39,017 Da

ncbi pathways :

Adaptive Immune System Pathway (1269171); Antigen Processing-Cross Presentation Pathway (1269195); Class I MHC Mediated Antigen Processing Presentation Pathway (1269192); Cross-presentation Of Particulate Exogenous Antigens (phagosomes) Pathway (1269196); Immune System Pathway (1269170); Leishmaniasis Pathway (144181); Leishmaniasis Pathway (144028); Leukocyte Transendothelial Migration Pathway (83083); Leukocyte Transendothelial Migration Pathway (494); Osteoclast Differentiation Pathway (193147)

ncbi summary :

The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

uniprot summary :

p40phox: a component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst. Upon neutrophil stimulation, this protein and other cytosolic elements are sent to the cell membrane from the cytosol to form a complex which produces phagocytic oxygen radicals. Responsible for the downregulation of NADPH-oxidase. Alternative splicing has been observed. Protein type: Oxidoreductase. Chromosomal Location of Human Ortholog: 22q13.1. Cellular Component: cytosol; endosome membrane; membrane; NADPH oxidase complex. Molecular Function: phosphatidylinositol 3-phosphate binding; protein binding; protein dimerization activity; superoxide-generating NADPH oxidase activator activity. Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; cell redox homeostasis; immune response; positive regulation of catalytic activity; vascular endothelial growth factor receptor signaling pathway. Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type Iii

size1 :

0.1 mg

price1 :

300 USD

more info or order :

MyBioSource product webpage