catalog number :
MBS421419
products full name :
Goat anti-APPL1 Antibody
products short name :
APPL1
products name syn :
APPL1; DIP13alpha; adaptor protein containing pH domain; PTB domain and leucine zipper motif; signaling adaptor protein DIP13alpha; Goat Anti-APPL / DIP13alpha; DIP13alpha; adaptor protein containing pH domain, PTB domain and leucine zipper motif; signaling adaptor protein DIP13alpha
other names :
DCC-interacting protein 13-alpha; DCC-interacting protein 13-alpha; DCC-interacting protein 13-alpha; dip13-alpha; AKT2 interactor; signaling adaptor protein DIP13alpha; adapter protein containing PH domain, PTB domain and leucine zipper motif 1; adaptor protein containing pH domain, PTB domain and leucine zipper motif 1; adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1; Adapter protein containing PH domain, PTB domain and leucine zipper motif 1
products gene name :
APPL1
other gene names :
APPL1; APPL1; APPL; DIP13alpha; ; Dip13-alpha
uniprot entry name :
DP13A_HUMAN
reactivity :
Human, Mouse, Rat, Dog, Cow
sequence :
NRYSRLSKKRENDK
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Western Blot (WB)
app notes :
Western Blot: Approx 80kDa band observed in Rat Aortic Smooth Skeletal Muscle Cells (lane 1), intensity increased after infection with Human APPL1 adenovirus for 48h (lane 2). Calculated MW of 79.7kDa according to NP_036228.1.
The blot was co-labeled with Tubulin-specific antibodies as a loading control. Recommended concentration: 0.3-1ug/ml. Peptide ELISA: antibody detection limit dilution 1:64000.
other info1 :
Epitope: Internal Region. Immunogen: Peptide with sequence C-NRYSRLSKKRENDK, from the internal region of the protein sequence according to NP_036228.1.
other info2 :
Corresponding Immunizing Peptide: Catalog #MBS426327
ncbi acc num :
NP_036228.1
ncbi gb acc num :
NM_012096.2
ncbi mol weight :
79,663 Da
ncbi pathways :
Apoptosis Pathway 105648!!Colorectal Cancer Pathway 83106!!Colorectal Cancer Pathway 518!!Coregulation Of Androgen Receptor Activity Pathway 138085!!EGFR1 Signaling Pathway 198782!!FSH Signaling Pathway 672455!!Pathways In Cancer 83105!!Regulation Of Apoptosis Pathway 105685!!Role Of DCC In Regulating Apoptosis Pathway 161011
ncbi summary :
The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq, Jul 2008]