catalog number :
MBS421418
products full name :
Goat anti-GCIP / MAID Antibody
products short name :
GCIP/MAID
products name syn :
CCNDBP1; cyclin D-type binding-protein 1; DIP1; GCIP; HHM Protein; MAID protein; grap2 cyclin interacting protein; D-type cyclin-interacting protein 1; Goat Anti-Cyclin D-type binding-protein 1 (aa322-333); CCNDBP1; cyclin D-type binding-protein 1; DIP1; GCIP; HHM Protein; MAID protein; grap2 cyclin interacting protein; D-type cyclin-interacting protein 1
other names :
cyclin-D1-binding protein 1; Cyclin-D1-binding protein 1; cyclin-D1-binding protein 1; human homolog of Maid; D-type cyclin-interacting protein 1; grap2 and cyclin-D-interacting protein; cyclin D-type binding-protein 1; Grap2 and cyclin-D-interacting protein; Human homolog of Maid
products gene name :
CCNDBP1
other gene names :
CCNDBP1; CCNDBP1; HHM; DIP1; GCIP; DIP1; GCIP; HHM
uniprot entry name :
CCDB1_HUMAN
purity :
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
form :
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
concentration :
100ug specific antibody in 200ul
storage stability :
Aliquot and store at -20 degree C. Minimize freezing and thawing.
tested application :
Peptide ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
app notes :
Immunohistochemistry: In paraffin embedded Human Prostate shows textured cytoplasm staining with emphasis on the luminal side of the secretory cells in the glands. Recommended concentration, 3-6ug/ml. Western Blot: Preliminary experiments gave an approx 55kDa band in K562 lysate at 0.5ug/ml. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 41kDda and 27kDa according to NP_036274; NP_411241. Peptide ELISA: antibody detection limit dilution 1:32000.
other info1 :
Epitope: C Terminus. Immunogen: Peptide with sequence C-NRIKELTQSELEL, from the C Terminus of the protein sequence according to NP_036274.3; NP_411241.1. Corresponding Immunizing Peptide: Catalog #MBS428342
other info2 :
Important Note: This antibody is expected to recognise both human isoforms of this protein.
ncbi acc num :
NP_036274.3
ncbi gb acc num :
NM_012142.4
ncbi mol weight :
22,714 Da
ncbi summary :
This gene was identified by the interaction of its gene product with Grap2, a leukocyte-specific adaptor protein important for immune cell signaling. The protein encoded by this gene was shown to interact with cyclin D. Transfection of this gene in cells was reported to reduce the phosphorylation of Rb gene product by cyclin D-dependent protein kinase, and inhibit E2F1-mediated transcription activity. This protein was also found to interact with helix-loop-helix protein E12 and is thought to be a negative regulator of liver-specific gene expression. Several alternatively spliced variants have been found for this gene. [provided by RefSeq, Apr 2009]