antibody

Goat anti-MBD2 (isoform 1) Antibody

MBS421404

0.1 mg

290 USD

polyclonal

goat

nonconjugated

human, mouse, rat, dog

western blot, immunohistochemistry, enzyme immunoassay

Antibody

Goat anti-MBD2 (isoform 1) Antibody

MBD2

MBD2; methyl-CpG binding domain protein 2; DKFZp586O0821; DMTase; NY-CO-41; MBD2; methyl-CpG binding domain protein 2; DKFZp586O0821; DMTase; NY-CO-41

methyl-CpG-binding domain protein 2 isoform 1; Methyl-CpG-binding domain protein 2; methyl-CpG-binding domain protein 2; demethylase; methyl-CpG binding domain protein 2; Demethylase; DMTase; Methyl-CpG-binding protein MBD2

MBD2

MBD2; MBD2; DMTase; NY-CO-41; DMTase

MBD2_HUMAN

Polyclonal

Goat

Human, Mouse, Rat, Dog

411

RNDPLNQNKGKPDLN

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100ug specific antibody in 200ul

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

Immunohistochemistry: In paraffin embedded Human Colon shows nuclear stianing of enterocytes. Recommended concentration, 2-4ug/ml. Western Blot: Approx 48-50kDa band observed in lysates of cell line Jurkat (calculated MW of 43.3kDa according to NP_003918.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Tan et al, Mol Cell Biol. 2006 Oct;26(19):7224-35.; PMID: 16980624). Recommended concentration: 0.3-1ug/ml. Peptide ELISA: antibody detection limit dilution 1:64000.

Epitope: Internal Region. Immunogen: Peptide with sequence C-RNDPLNQNKGKPDLN, from the internal region of the protein sequence according to NP_003918.1. Corresponding Immunizing Peptide: Catalog #MBS425651

Important Note: This antibody is expected to resognize isoform 1 (NP_003918.1) only.

4505117

NP_003918.1

NM_003927.4

31,745 Da

Epigenetic Regulation Of Gene Expression Pathway 980449!!Gene Expression Pathway 105937!!Negative Epigenetic Regulation Of RRNA Expression Pathway 980452!!NoRC Negatively Regulates RRNA Expression Pathway 980454!!RNA Polymerase I Promoter Clearance Pathway 106551!!RNA Polymerase I Promoter Opening Pathway 106552!!RNA Polymerase I Transcription Pathway 106550!!RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway 160948!!Signaling Events Mediated By HDAC Class I Pathway 137997

DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]

0.1 mg

290 USD